Preeclampsia, lipid peroxidation, and calcium adenosine triphosphatase activity of red blood cell ghosts

      This paper is only available as a PDF. To read, Please Download here.
      Objective: We evaluated the effect of lipid peroxidation on the calcium adenosine triphosphatase activity of red blood cell ghosts from normotensive pregnant women and compared it with the adenosine triphosphatase activity and lipid peroxidation in preeclampsia.
      Study Design: Ten nulliparous normotensive and 10 nulliparous preeclamptic pregnant women (38 to 39 weeks of gestation) were used as blood donors. Preeclampsia was diagnosed on the basis of blood pressure (>140/90 mm Hg) and proteinuria (>0.5 gm of urinary protein per day). Red blood cell ghosts were prepared for both groups and used for calcium adenosine triphosphatase activity and lipid peroxidation determinations. Control ghosts (normotensive) were irradiated with ultraviolet light for different lengths of time.
      Results: Calcium adenosine triphosphatase activity of red blood cell ghosts from normotensive women is sensitive to lipid peroxidation. The lipid peroxidation of red blood cell ghosts from preeclamptic women is higher than that from normotensive women.
      Conclusion: The diminution of the calcium adenosine triphosphatase activity with preeclampsia could be explained by the sensitivity of this adenosine triphosphatase to lipid peroxidation.

      Key words

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to American Journal of Obstetrics & Gynecology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • National High Blood Pressure Education Program Working Group
        Report on high blood pressure during pregnancy.
        Am J Obstet Gynecol. 1990; 163: 1689-1712
        • Belizán JM
        • Villar J
        • Repke J
        The relationship between calcium intake and pregnancy-induced hypertension: up-to-date evidence.
        Am J Obstet Gynecol. 1988; 158: 898-902
        • Sowers JR
        • Zemel MB
        • Bronsteen RA
        • Zemel PC
        • Walsh MF
        • Standley PR
        • et al.
        Erythrocyte cation metabolism in preeclampsia.
        Am J Obstet Gynecol. 1989; 161: 441-445
        • Haller H
        • Oeney T
        • Hauck U
        • Destler A
        • Philipp T
        Increased intracellular free calcium and altered sensitivity to angiotensin II in platelets of preeclamptic women.
        in: International Soceity for the Study of Hypertension in Pregnancy, Montreal1988: 101
        • Nardulli G
        • Proverbio F
        • Limongi FG
        • Marín R
        • Proverbio T
        Preeclampsia and calcium adenosine triphosphatase activity of red blood cell ghosts.
        Am J Obstet Gynecol. 1994; 171: 1361-1365
        • Walsh SW
        Preeclampsia: an imbalance in placental prostacyclin and thromboxane production.
        Am J Obstet Gynecol. 1985; 152: 335-340
        • Halliwell B
        • Gutteridge JMC
        Free radicals in biology and medicine.
        Clarendon Press, Oxford (UK)1989
        • Jain SK
        • Wise R
        Relationship between elevated lipid peroxides, vitamin E deficiency and hypertension in preeclampsia.
        Mol Cell Biochem. 1995; 151: 33-38
        • Cueto SM
        • Romney AD
        • Wang Y
        • Walsh SW
        Beta-carotene attenuates peroxide-induced vasoconstriction in the human placenta.
        J Soc Gynecol Invest. 1997; 4: 64-71
        • Davidge ST
        • Hubel CA
        • Brayden RD
        • Capeless EC
        • McLaughlin MK
        Sera antioxidant activity in uncomplicated and preeclamptic pregnancies.
        Obstet Gynecol. 1992; 79: 897-901
        • Hubel CA
        • Roberts JM
        • Taylor RN
        • Musci TJ
        • Rogers GM
        • McLaughlin MK
        Lipid peroxidation in pregnancy: new perspectives on preeclampsia.
        Am J Obstet Gynecol. 1989; 161: 1025-1034
        • Snotnikova LG
        • Naumov AV
        • Kuznetsova VA
        The value of some parameters of erythrocyte membrane lipid peroxidation in late gestosis.
        Akush Ginekol (Mosk). 1986; 4: 20-22
        • Ohta A
        • Mohri T
        • Ohyashiki T
        Effect of lipid peroxidation on membrane-bound Ca2+-ATPase activity of the intestinal brushborder membranes.
        Biochim Biophys Acta. 1989; 984: 151-157
        • Marín R
        • Rodríguez AJ
        • Proverbio T
        Partial characterization of the inhibitory effect of lipid peroxidation on the ouabain-insensitive Na-ATPase of rat kidney cortex plasma membranes.
        J Bioenerg Biomembr. 1992; 24: 329-335
        • Heinz E
        • Hoffman JF
        Phosphate incorporation and Na,K-ATPase activity in human red blood cell ghosts.
        J Cell Comp Physiol. 1965; 65: 31-44
        • Marín R
        • Proverbio T
        • Proverbio F
        Inside-out basolateral plasma membrane vesicles from rat kidney proximal tubular cells.
        Biochim Biophys Acta. 1986; 858: 195-201
        • Feix JB
        • Bachowski GJ
        • Girotti AW
        Photodynamic action of merocyanine 540 on erythrocyte membranes: structural perturbation of lipid and protein constituents.
        Biochim Biophys Acta. 1991; 1075: 28-35
        • Folch J
        • Lees M
        • Sloane-Stanley GH
        A simple method for the isolation and purification of total lipids from animal tissues.
        J Biol Chem. 1957; 226: 497-509
        • Pryor WA
        • Castle L
        Chemical methods for the detection of lipid hydroperoxides.
        Methods Enzymol. 1984; 105: 293-299
        • Moore RB
        • Brummitt ML
        • Mankad VN
        Hydroperoxides selectively inhibit human erythrocyte membrane enzymes.
        Arch Biochem Biophys. 1989; 273: 527-534
        • Rajeswari P
        • Natarajan R
        • Nadler JL
        • Kumar D
        • Kalra VK
        Glucose induces lipid peroxidation and inactivation of membrane-associated ion-transport enzymes in human erythrocytes in vivo and in vitro.
        J Cell Physiol. 1991; 149: 100-109
        • Carafoli E
        Calcium pump of the plasma membrane.
        Physiol Rev. 1991; 71: 129-153
        • Hebbel RP
        • Shalev O
        • Foker W
        • Rank BH
        Inhibition of erythrocyte Ca2+-ATPase by activated oxygen through thiol- and lipid-dependent mechanisms.
        Biochim Biophys Acta. 1986; 862: 8-16
        • Ramsey EM
        • Donna MW
        Placental vasculature and circulation.
        in: WB Saunders, Philadelphia1980: 1-101
        • Yoshikawa T
        • Furukawa Y
        • Wakamatsu Y
        • Takemura S
        • Tanaka H
        • Kondo M
        Experimental hypoxia and lipid peroxide in rats.
        Biochem Med. 1982; 27: 207-213