Abstract
OBJECTIVE: The purpose of this study was to determine the accuracy and feasibility of sequential
polar body removal and analysis for preimplantation genetic diagnosis of mendelian
disorders. STUDY DESIGN: Three couples with risk factors for cystic fibrosis had preimplantation genetic diagnosis
with the use of sequential polar body analysis. After stimulation, oocytes were harvested
and the first polar bodies were removed and analyzed on the day of aspiration. The
following day, after fertilization, the second polar bodies were aspirated. Only embryos
known to have inherited the normal maternal allele were transferred. RESULTS: All three couples had successful pregnancies resulting in the births of unaffected
infants. CONCLUSIONS: Preimplantation diagnosis with the use of sequential polar body removal is feasible
and can prevent the establishment of genetically abnormal pregnancies for couples
at risk. (Am J Obstet Gynecol 1998;178:1298-306.)
Keywords
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Article info
Footnotes
☆From the Reproductive Genetics Institute, Department of Obstetrics and Gynecology, Illinois Masonic Medical Center.
☆☆Reprint requests: Charles M. Strom, MD, Department of Obstetrics and Gynecology, Reproductive Genetics Institute, Illinois Masonic Medical Center, 836 Wellington Ave., Chicago IL 60657.
★6/6/90062
Identification
Copyright
© 1998 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
