Advertisement

Menstrual cycle–associated regulation of anabolic and catabolic enzymes causes luteal phase–characteristic expression of sulfatide in human endometrium

      Abstract

      OBJECTIVE: Our purpose was to investigate the metabolic background of the expression of sulfoglycolipids in human endometrium during the luteal phase. STUDY DESIGN: We investigated the expression of sulfoglycolipids by thin-layer chromatography immunostaining and the activities of galactosylceramide sulfotransferase and arylsulfatase A, which regulate the synthesis and degradation of sulfoglycolipid. In addition, arylsulfatase A messenger ribonucleic acid was studied by reverse transcriptase polymerase chain reaction. RESULTS: Sulfoglycolipid expression showed a marked increase in the luteal phase but not in the follicular phase, whereas sialoglycolipids remained relatively constant. The increase of sulfoglycolipids was found to be due to 4.5-fold increased activation of sulfotransferase and a concurrent reduction of arylsulfatase A activity in the luteal phase. Arylsulfatase A messenger ribonucleic acid was detected in both phases and showed no significant changes. CONCLUSIONS: These findings suggest that increased sulfoglycolipid expression in the luteal phase is due to the simultaneous regulation of sulfotransferase and arylsulfatase A, probably by sex steroid hormones. (Am J Obstet Gynecol 1997;176:142-9.)

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to American Journal of Obstetrics & Gynecology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Hakomori S
        Tumor-associated carbohydrate antigens.
        Annu Rev Immunol. 1984; 2: 103-126
        • Saitoh T
        • Natomi H
        • Zhao W
        • Okuzumi K
        • Sugano K
        • Iwamori M
        • et al.
        Identification of glycolipid receptors for Helicobacter pylori by TLC-immunostaining.
        FEBS Lett. 1991; 282: 385-387
        • Bhat S
        • Spitalnik S
        • Gonzalez-Scarano F
        • Silverberg DH
        Galactosyl ceramide or a derivative is an essential components of the neural receptor for HIV-1 envelope glycoprotein gp120.
        Proc Natl Acad Sci U S A. 1991; 88: 7131-7134
        • Mohan PS
        • Laitinen J
        • Merenmies J
        • Rauvala H
        • Jungalwala FB
        Sulfoglycolipids bind to adhesive protein amphoterin (P30) in the nervous system.
        Biochem Biophys Res Commun. 1992; 182: 689-696
        • Tanphaichitr N
        • Smith J
        • Mongkolsirikieart S
        • Gradil C
        • Lingwood CA
        Role of gamete-specific sulfoglycolipid immobilizing protein on mouse sperm-egg binding.
        Dev Biol. 1993; 156: 164-175
        • Kubushiro K
        • Kojima K
        • Mikami M
        • Nozawa S
        • Iizuka R
        • Iwamori M
        • et al.
        Menstrual cycle–associated alteration of sulfogalactosylceramide in human uterine endometrium: possible induction of glycolipid sulfation by sex steroid hormones.
        Arch Biochem Biophys. 1989; 268: 129-136
        • Takamatsu K
        • Kamei K
        • Kubushiro K
        • Kiguchi K
        • Nozawa S
        • Iwamori M
        Luteal phase–characteristic induction of I3SO3-GalCer in human cervical epithelia and uterine endometria, and follicular phase–characteristic formation of a ganglioside-derived negative charge gradient in different regions of fallopian tubes.
        Biochem Biophys Acta. 1993; 1170: 232-236
        • Roberts DD
        • Ginsberg V
        Sulfated glycolipids and cell adhesion.
        Arch Biochem Biophys. 1988; 267: 405-415
        • Aruffo A
        • Kolanus W
        • Walz G
        • Fredman P
        • Seed B
        CD62/P-selectin recognition of myeloid and tumor cell sulfatides.
        Cell. 1991; 67: 35-44
        • Erbe D
        • Watson SR
        • Presta LG
        • Wolitzky BA
        • Foxall C
        • Brandley BK
        • et al.
        P- and E-selectin use common sites for carbohydrate ligand recognition and cell adhesion.
        J Cell Biol. 1993; 120: 1227-1235
        • Kobayashi T
        • Honke K
        • Kuramitsu Y
        • Hosokawa M
        • Miyazaki T
        • Murata J
        • et al.
        Cell-surface sulfoglycolipids are involved in the attachment of renal-cancer cells to laminin.
        Int J Cancer. 1994; 56: 281-285
        • Stein C
        • Gieselmann V
        • Kreysing J
        • Schmidt B
        • Pohlmann R
        • Waheed A
        • et al.
        Cloning and expression of human arylsulfatase A.
        J Biol Chem. 1989; 264: 1252-1259
        • Iwamori M
        • Sawada K
        • Hara Y
        • Nishio M
        • Fujisawa T
        • Imura H
        • et al.
        Neutral glycosphingolipids and gangliosides of bovine thyroid.
        J Biochem. 1982; 91: 1875-1887
        • Kohler G
        • Milstein C
        Continuous cultures of fused cells secreting antibody of predefined specificity.
        Nature. 1975; 256: 495-497
        • Fredman P
        • Mattsson L
        • Andersson K
        • Davidsson P
        • Ishizuka I
        • Jeansson S
        • et al.
        Characterization of the binding epitope of a monoclonal antibody to sulphatide.
        Biochem J. 1988; 251: 17-22
        • Iwamori M
        • Sunada S
        • Ishihara E
        • Moki M
        • Fujimoto S
        • Nagai Y
        Differential expression of Fucosyl GM1 and a disialoganglioside with a NeuAc α 2-6 GalNAc linkage in various rat ascites hepatoma cells.
        FEBS Lett. 1986; 198: 66-70
        • Bradford MM
        Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
        Anal Biochem. 1976; 72: 248-254
        • Harinath BC
        • Robins E
        Arylsulfatases in human brain: assay, some properties, and distribution.
        J Neurochem. 1971; 18: 237-244
        • van der Pal RHM
        • Klein W
        • van Golde LMG
        • Lopes CM
        Developmental profiles of arylsulfatases A and B in rat cerebral cortex and spinal cord.
        Biochem Biophys Acta. 1991; 1081: 315-320
        • Baum H
        • Dodgson KS
        • Spencer B
        The assay of arylsulfatase A and B in human urine.
        Clin Chim Acta. 1959; 4: 453-455
        • Natomi H
        • Saitoh T
        • Sugano K
        • Iwamori M
        • Fukayama M
        • Nagai Y
        Systematic analysis of glycosphingolipids in the human gastrointestinal tract: enrichment of sulfatides with hydroxylated longer-chain fatty acids in the gastric and duodenal mucosa.
        Lipids. 1993; 28: 737-742
        • Roberts DD
        • Wewer UM
        • Liotta LA
        • Ginsburg V
        Laminin-dependent and laminin-independent adhesion of human melanoma cells to sulfatides.
        Cancer Res. 1988; 48: 3367-3373
        • Turpeenniemi-Hujanen T
        • Ronnberg L
        • Kauppila A
        • Puistola U
        Laminin in the human embryo implantation: analogy to the invasion by malignant cells.
        Fertil Steril. 1992; 58: 105-113
        • Momoeda M
        • Taketani Y
        • Mizuno M
        • Iwamori M
        • Nagai Y
        Characteristic expression of cholesterol sulfate in rabbit endometrium during the implantation period.
        Biochem Biophys Res Commun. 1991; 178: 145-150
        • Momoeda M
        • Cui Y
        • Sawada Y
        • Taketani Y
        • Mizuno M
        • Iwamori M
        Pseudopregnancy-dependent accumulation of cholesterol sulfate due to up-regulation of cholesterol sulfotransferase and concurrent down-regulation of cholesterol sulfate sulfatase in the uterine endometria of rabbits.
        J Biochem. 1994; 116: 657-662