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Angiotensin-converting enzyme: Serum levels during normal pregnancy

  • Joaquim Vaz Parente
    Correspondence
    Department of Gynecology, Obstetrics and Pediatrics, Research Laboratory, Hospital das Clinicas, Faculty of Medicine of Ribeirão Preto, 14.100 Ribeirão Preto, São Paulo, Brazil
    Affiliations
    From the Department of Gynecology, Obstetrics and Pediatrics, Research Laboratory, Hospital das Clinicas, and Department of Biochemistry, Protein Chemistry Laboratory, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • José Gonçalves Franco Jr.
    Affiliations
    From the Department of Gynecology, Obstetrics and Pediatrics, Research Laboratory, Hospital das Clinicas, and Department of Biochemistry, Protein Chemistry Laboratory, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Lewis Joel Greene
    Affiliations
    From the Department of Gynecology, Obstetrics and Pediatrics, Research Laboratory, Hospital das Clinicas, and Department of Biochemistry, Protein Chemistry Laboratory, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Roberto Salles Meirelles
    Affiliations
    From the Department of Gynecology, Obstetrics and Pediatrics, Research Laboratory, Hospital das Clinicas, and Department of Biochemistry, Protein Chemistry Laboratory, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Alberto Raul Martinez
    Affiliations
    From the Department of Gynecology, Obstetrics and Pediatrics, Research Laboratory, Hospital das Clinicas, and Department of Biochemistry, Protein Chemistry Laboratory, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
    Search for articles by this author
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      ACE activity of the serum of 52 normal pregnant women was measured in vitro under conditions of substrate saturation with Hip-His-Leu as substrate. The product His-Leu was measured by fluorimetry after reaction with o-phthaldehyde. ACE activity (nmol/min/ml serum) was 30.6±7.8, 28.8±7.4, and 30.9±8.2 for the first, second, and third trimester of pregnancy, respectively. No statistically significant differences (p>0.05) in ACE activity were detected among the three trimesters of normal pregnancy with either serum volume or serum protein as reference value. These values are within the range reported by Friedland and Silverstein13 for 51 male and seven female healthy blood bank donors. We conclude that the evolution of normal pregnancy does not significantly modify the levels of ACE in peripheral blood serum.
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