A simple way to determine nonspecific effects of plasma and serum components in radioreceptor assays and radioimmunoassays for human chorionic gonadotropin

      This paper is only available as a PDF. To read, Please Download here.
      A simple approach is validated for the determination of nonspecific effects of human plasma and serum in the radioreceptor assays and radioimmunoassays for human chorionic gonadotropin (hCG). The approach is based on the findings that, despite differences in the degree of inhibition of 125I-hCG binding to its receptors and antibodies by nonhormonal components in different dilutions of pool plasma and serum, the standard curves (plotted as the percentage of control for each serum or plasma dilution) are superimposable. The approach consists of (1) running a single standard curve with no pool plasma or serum, (2) including a set of “correction” tubes which contain pool plasma or serum diluted correspondingly to the dilution of the unknown samples in the assay, (3) dividing the counts per minute found in the correction tubes into the counts per minute of the unknown samples and multiplying by 100, and (4) using this value to obtain the amount of hCG in the unknown samples by comparison with the no pool plasma or serum standard curve.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to American Journal of Obstetrics & Gynecology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Lee C.Y.
        • Ryan R.J.
        Radioreceptorassay for human chorionic gonadotropin.
        J. Clin. Endocrinol. Metab. 1975; 40: 228
        • Catt K.J.
        • Dufau M.L.
        • Vaitukaitis J.L.
        Appearance of hCG in pregnancy plasma following the initiation of implantation of the blastocyst.
        J. Clin. Endocrinol. Metab. 1975; 40: 537
        • Leidenberger F.A.
        • Willaschek R.
        • Pahnke V.G.
        • Reichert Jr., L.E.
        Application of radioligand receptor assay for determination of luteinizing hormone in human serum.
        Acta Endocrinol. 1976; 81: 54
        • Schmidt-Gollwitzer M.
        • Eiletz J.
        • Sackmann U.
        • Nevinnev-Stickel J.
        Detection of ovulation by a radioreceptorassay for luteinizing hormone.
        J. Clin. Endocrinol. Metab. 1978; 47: 902
        • Vaitukaitis J.L.
        • Braunstein G.D.
        • Ross G.T.
        A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone.
        Am. J. Obstet. Gynecol. 1972; 113: 751
        • Hunter W.M.
        • Bennie J.G.
        Reduction of nonspecific serum responses in human pituitary gonadotropin radioimmunoassays.
        J. Endocrinol. 1979; 80: 59
        • Schams D.
        • Menzer C.
        Determination of luteinizing hormone in bovine blood by radioligand receptor assay and comparison with radioimmunological evaluation.
        Acta Endocrinol. 1978; 87: 268
        • Lefkowitz R.J.
        • Roth J.
        • Pastan I.
        Radioreceptorassay of adrenocorticotropic hormone: new approach to assay of polvpeptide hormones in plasma.
        Science. 1970; 170: 633
        • Sonksen P.H.
        Non-specific interference in radioimmunoassay systems.
        J. Endocrinol. 1972; 54: 5
        • Herington A.C.
        • Jacobs L.S.
        • Daughaday W.H.
        Radioreceptor and radioimmunossay quantitation of human growth hormone in acromegalic serum: overestimation by immunoassay and systematic differences between antisera.
        J. Clin. Endocrinol. Metab. 1971; 39: 257
        • Cheng K.W.
        A radioreceptorassay for follicle stimulating hormone.
        J. Clin. Endocrinol. Metab. 1975; 41: 581
        • Spencer C.A.
        • Challand G.S.
        Interference in a radioimmunoassay for human thyrotropin.
        Clin. Chem. 1977; 23: 584
        • Miyachi Y.
        • Vaitukaitis J.L.
        • Neischlag F.
        • Lipsett M.B.
        Enzymatic radioiodination of gonadotropins.
        J. Clin. Endocrinol. Metab. 1972; 34: 23
        • Rao Ch.V.
        • Griffin L.P.
        • Carman Jr., F.R.
        Gonadotropin receptors in human corpora lutea of the menstrual cycle and pregnancy.
        Am. J. Obstet. Gynecol. 1977; 128: 146
        • Rao Ch.V.
        Properties of gonadotropin receptors in the cell membranes of bovine corpus luteum.
        J. Biol. Chem. 1974; 249: 2864
        • Hussa R.O.
        Effects of antimicrotubule agents, potassium and inhibitors of energy production on hCG secretion.
        In Vitro. 1979; 15: 237
        • Braunstein G.D.
        • Kamadar V.
        • Rasor J.
        • Swaminathan N.
        • Wade M.E.
        Widespread distribution of chorionic gonadotropin like substances in normal human tissues.
        J. Clin. Endocrinol. Metab. 1979; 49: 917
        • Hussa R.O.
        • Mattingly R.F.
        Semiquantitative use of a Biocept-G radioreceptorassay.
        Obstet. Gynecol. 1979; 54: 199
        • Steel R.G.
        • Torrie J.H.
        Principles and Procedures of Statistics. McGraw-Hill Book Co., Inc, New York1960