Poster session II Clinical obstetrics, diabetes, labor, medical-surgical-disease, physiology/endocrinology, prematurity: Abstracts 237 - 386| Volume 208, ISSUE 1, SUPPLEMENT , S125, January 01, 2013

274: Association of the Pro12Ala polymorphism of PPARγ2 with mild gestational hyperglycemia and gestational diabetes


      To determine the prevalence of the Pro12Ala polymorphism in pregnant women with normoglycemia, mild gestational hyperglycemia, and gestational diabetes (GDM).

      Study Design

      Diagnosis of GDM was made using the 100g oral glucose tolerance test according to the ADA criteria. Gestational hyperglycemia was diagnosed using the glycemic profile test, during which a standardized 2,840 calorie diet is administered across five meals, with measurement of glucose levels fasting and two hours following each meal. Mild gestational hyperglycemia was diagnosed with one or more abnormal values of fasting glucose >90 mg/dL or postprandial glucose >130 mg/dL. Genotyping for the Pro12Ala allele was performed on DNA from maternal serum samples by the RFLP method using the BstU1 restriction endonuclease. Patients were classified as Pro/Pro homozygotes (single 244 base pair (bp) segment), Pro/Ala heterozygotes (244, 223, and 21 bp segments), or Ala/Ala homozygotes (223 and 21 bp segments). Genotype frequencies were compared between groups using the Chi-Square test with p<0.05 for statistical significance.


      One hundred fifty-one pregnant women were included in the study. Fifty-four women were normoglycemic, 32 had mild gestational hyperglycemia, and 65 were classified as GDM. There were no Ala/Ala homozygotes in any group. The frequency of Pro/Ala heterozygotes was significantly lower in women with mild gestational hyperglycemia relative to normoglycemic women (9.4% vs. 17.3%, p<0.05), and in women with GDM relative to normoglycemic women (10.9% vs. 17.3%, p<0.05). The frequency of heterozygotes did not differ between patients with mild gestational hyperglycemia and GDM.


      The prevalence of Pro12Ala was higher among normoglycemic women, suggesting that this polymorphism may be protective against hyperglycemia in pregnancy. Further investigation is needed to elucidate the effect of this polymorphism on adverse maternal and neonatal outcomes associated with these conditions.