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Poster session I Clinical obstetrics, epidemiology, fetus, medical-surgical complications, neonatalogy, physiology/endocrinology, prematurity: Abstracts 87 - 236| Volume 208, ISSUE 1, SUPPLEMENT , S52, January 01, 2013

88: Deregulated cytokine and chemokind expression in endometrium from women with recurrent pregnancy loss

      Objective

      Implantation in humans is a complex process that is temporally and spatially restricted. Using a one-by-one approach, several gene products that may participate in this process have been identified. Our objective was to explore if those genes are differentially expressed in endometrium from women with recurrent pregnancy loss (RPL).

      Study Design

      Endometrial samples were obtained through hysteroscopic biopsy from 5 patients with 2 or more consecutive pregnancy losses and 5 women with previous normal pregnancy, matched for age, menstrual period and body mass index. Exclusion criteria were chronic diseases, autoimmune and connective tissue disorders, or cancer. A RT2 Profiler PCR Array System - Human Cytokines & Chemokines (PAHS - 150D) was used to profile the expression of 96 genes in RPL samples and controls. Data were confirmed by quantitative real-time PCR and western blot. Significance was set at p<0.05.

      Results

      In women with RPL, 4 genes were significantly up-regulated (PPBP, BMP2 and 7, CCL21), and 28 significantly down-regulated. The proinflammatory chemokines BMP2 and CCL21 were found up-regulated. Significant downregulation was detected for several genes involved in angiogenesis (IL22,IL23), interleukines implicated in Th1/Th2 balance (IL4, IL5) and CSF1, which is involved in the development of placenta.

      Conclusion

      The gene expression profile of the endometrium of women with RPL, obtained through PCR array technology, is consistent with a proinflammation state, a shift fromTh1/Th2, a decreased angiogenesis and an improper placental development.
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