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Human amnion epithelial cells reduce ventilation-induced preterm lung injury in fetal sheep

Published:March 09, 2012DOI:https://doi.org/10.1016/j.ajog.2012.02.038

      Objective

      The objective of the study was to explore whether human amnion epithelial cells (hAECs) can mitigate ventilation-induced lung injury.

      Study Design

      An established in utero ovine model of ventilation-induced lung injury was used. At day 110 of gestation, singleton fetal lambs either had sham in utero ventilation (IUV) (n = 4), 12 hours of IUV alone (n = 4), or 12 hours of IUV and hAEC administration (n = 5). The primary outcome, structural lung injury, was assessed 1 week later.

      Results

      Compared with sham controls, IUV alone was associated with significant lung injury: increased collagen (P = .03), elastin (P = .02), fibrosis (P = .02), and reduced secondary-septal crests (P = .009). This effect of IUV was significantly mitigated by the administration of hAECs: less collagen (P = .03), elastin (P = .04), fibrosis (P = .02), normalized secondary-septal crests (P = .02). The hAECs were immunolocalized within the fetal lung and had differentiated into type I and II alveolar cells.

      Conclusion

      The hAECs mitigate ventilation-induced lung injury and differentiated into alveolar cells in vivo.

      Key words

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