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Differential expression of smooth muscle regulatory proteins in the uterosacral ligaments of women with uterine prolapse

Published:April 22, 2010DOI:https://doi.org/10.1016/j.ajog.2010.02.053

      Objective

      To compare smooth muscle regulatory protein expression in the uterosacral ligament (USL) of women with and without uterine prolapse.

      Study Design

      USLs ligament were sampled in women with (n = 9) or without (n = 9) uterine prolapse. Caldesmon, smooth muscle actin (SMA), myosin heavy chain, and zinc finger protein messenger RNA expression was assessed by quantitative real-time polymerase chain reaction. Immunohistochemistry and digital image analysis were used to determine protein expression.

      Results

      Caldesmon messenger RNA expression and the ratio of caldesmon-SMA messenger RNA expression was significantly increased in the USL from women with uterine prolapse compared with women without prolapse (caldesmon mean ± standard deviation messenger RNA, 0.81 ± 0.46 vs 0.39 ± 0.16; P = .01 and caldesmon-SMA messenger RNA ratio, mean ± standard deviation, 0.11 ± 0.04 vs 0.07 ± 0.02; P = .01). In addition, the ratio of caldesmon-SMA staining was significantly increased in women with uterine prolapse compared with women without prolapse (mean ± standard deviation, 0.44 ± 0.28 vs 0.28 ± 0.16; P = .03).

      Conclusion

      Uterine prolapse is associated with an increased ratio of caldesmon-SMA actin expression.

      Key words

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      References

        • Nygaard I.
        • Barber M.D.
        • Burgio K.L.
        • et al.
        Prevalence of symptomatic pelvic floor disorders in US women.
        JAMA. 2008; 300: 1311-1316
        • Olsen A.L.
        • Smith V.J.
        • Bergstrom J.O.
        • Colling J.C.
        • Clark A.L.
        Epidemiology of surgically managed pelvic organ prolapse and urinary incontinence.
        Obstet Gynecol. 1997; 89: 501-506
        • Chen B.H.
        • Wen Y.
        • Li H.
        • Polan M.L.
        Collagen metabolism and turnover in women with stress urinary incontinence and pelvic prolapse.
        Int Urogynecol J Pelvic Floor Dysfunct. 2002; 13 (discussion 87): 80-87
        • Smith A.R.
        • Hosker G.L.
        • Warrell D.W.
        The role of partial denervation of the pelvic floor in the aetiology of genitourinary prolapse and stress incontinence of urine.
        BJOG. 1989; 96: 24-28
        • Boreham M.K.
        • Miller R.T.
        • Schaffer J.I.
        • Word R.A.
        Smooth muscle myosin heavy chain and caldesmon expression in the anterior vaginal wall of women with and without pelvic organ prolapse.
        Am J Obstet Gynecol. 2001; 185: 944-952
        • Boreham M.K.
        • Wai C.Y.
        • Miller R.T.
        • Schaffer J.I.
        • Word R.A.
        Morphometric properties of the posterior vaginal wall in women with pelvic organ prolapse.
        Am J Obstet Gynecol. 2002; 187 (discussion 1508-9): 1501-1508
        • Boreham M.K.
        • Wai C.Y.
        • Miller R.T.
        • Schaffer J.I.
        • Word R.A.
        Morphometric analysis of smooth muscle in the anterior vaginal wall of women with pelvic organ prolapse.
        Am J Obstet Gynecol. 2002; 187: 56-63
        • Gabriel B.
        • Denschlag D.
        • Gobel H.
        • et al.
        Uterosacral ligament in postmenopausal women with or without pelvic organ prolapse.
        Int Urogynecol J Pelvic Floor Dysfunct. 2005; 16: 475-479
        • Gabriel B.
        • Watermann D.
        • Hancke K.
        • et al.
        Increased expression of matrix metalloproteinase 2 in uterosacral ligaments is associated with pelvic organ prolapse.
        Int Urogynecol J Pelvic Floor Dysfunct. 2006; 17: 478-482
        • Takacs P.
        • Gualtieri M.
        • Nassiri M.
        • Candiotti K.
        • Medina C.A.
        Vaginal smooth muscle cell apoptosis is increased in women with pelvic organ prolapse.
        Int Urogynecol J Pelvic Floor Dysfunct. 2008; 19: 1550-1564
        • Takacs P.
        • Gualtieri M.
        • Nassiri M.
        • Candiotti K.
        • Fornoni A.
        • Medina C.A.
        Caldesmon expression is decreased in women with anterior vaginal wall prolapse: a pilot study.
        Int Urogynecol J Pelvic Floor Dysfunct. 2009; 20: 985-990
        • Takacs P.
        • Nassiri M.
        • Gualtieri M.
        • Candiotti K.
        • Medina C.A.
        Uterosacral ligament smooth muscle cell apoptosis is increased in women with uterine prolapse.
        Reprod Sci. 2009; 16: 447-452
        • DeLancey J.O.
        Anatomic aspects of vaginal eversion after hysterectomy.
        Am J Obstet Gynecol. 1992; 166 (discussion 1724-8): 1717-1724
        • Buller J.L.
        • Thompson J.R.
        • Cundiff G.W.
        • Krueger Sullivan L.
        • Schon Ybarra M.A.
        • Bent A.E.
        Uterosacral ligament: description of anatomic relationships to optimize surgical safety.
        Obstet Gynecol. 2001; 97: 873-879
        • Hodgkinson J.L.
        Actin and the smooth muscle regulatory proteins: a structural perspective.
        J Muscle Res Cell Motil. 2000; 21: 115-130
        • Huber P.A.
        Caldesmon.
        Int J Biochem Cell Biol. 1997; 29: 1047-1051
        • Katsuyama H.
        • Wang C.L.
        • Morgan K.G.
        Regulation of vascular smooth muscle tone by caldesmon.
        J Biol Chem. 1992; 267: 14555-14558
        • Ansari S.
        • Alahyan M.
        • Marston S.B.
        • El-Mezgueldi M.
        Role of caldesmon in the Ca2+ regulation of smooth muscle thin filaments: evidence for a cooperative switching mechanism.
        J Biol Chem. 2008; 283: 47-56
        • Li Y.
        • Lin J.L.
        • Reiter R.S.
        • Daniels K.
        • Soll D.R.
        • Lin J.J.
        Caldesmon mutant defective in Ca(2+)-calmodulin binding interferes with assembly of stress fibers and affects cell morphology, growth and motility.
        J Cell Sci. 2004; 117: 3593-3604
        • Mannikarottu A.S.
        • Changolkar A.K.
        • Disanto M.E.
        • Wein A.J.
        • Chacko S.
        Over expression of smooth muscle thin filament associated proteins in the bladder wall of diabetics.
        J Urol. 2005; 174: 360-364
        • Kanematsu A.
        • Ramachandran A.
        • Adam R.M.
        GATA-6 mediates human bladder smooth muscle differentiation: involvement of a novel enhancer element in regulating alpha-smooth muscle actin gene expression.
        Am J Physiol Cell Physiol. 2007; 293: C1093-C1102
        • Santiago F.S.
        • Ishii H.
        • Shafi S.
        • et al.
        Yin Yang-1 inhibits vascular smooth muscle cell growth and intimal thickening by repressing p21WAF1/Cip1 transcription and p21WAF1/Cip1-Cdk4-cyclin D1 assembly.
        Circ Res. 2007; 101: 146-155
        • Bai S.W.
        • Chung D.J.
        • Yoon J.M.
        • Shin J.S.
        • Kim S.K.
        • Park K.H.
        Roles of estrogen receptor, progesterone receptor, p53 and p21 in pathogenesis of pelvic organ prolapse.
        Int Urogynecol J Pelvic Floor Dysfunct. 2005; 16: 492-496
        • Bump R.C.
        • Mattiasson A.
        • Bo K.
        • et al.
        The standardization of terminology of female pelvic organ prolapse and pelvic floor dysfunction.
        Am J Obstet Gynecol. 1996; 175: 10-17
        • Lehr H.A.
        • Mankoff D.A.
        • Corwin D.
        • Santeusanio G.
        • Gown A.M.
        Application of photoshop-based image analysis to quantification of hormone receptor expression in breast cancer.
        J Histochem Cytochem. 1997; 45: 1559-1565
        • Ozdegirmenci O.
        • Karslioglu Y.
        • Dede S.
        • et al.
        Smooth muscle fraction of the round ligament in women with pelvic organ prolapse: a computer-based morphometric analysis.
        Int Urogynecol J Pelvic Floor Dysfunct. 2005; 16 (discussion 43): 39-43
        • Lin A.D.
        • Levin R.M.
        • Kogan B.A.
        • et al.
        Alteration of contractile and regulatory proteins in estrogen-induced hypertrophy of female rabbit bladder.
        Urology. 2006; 68: 1139-1143
        • Yang L.
        • He D.L.
        • Wang S.
        • Cheng H.P.
        • Wang X.Y.
        Effect of long-term partial bladder outlet obstruction on caldesmon isoforms and their correlation with contractile function.
        Acta Pharmacol Sin. 2008; 29: 600-605