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Magnesium sulfate reduces inflammation-associated brain injury in fetal mice

  • Irina Burd
    Correspondence
    Reprints: Irina D. Burd, MD, PhD, Maternal and Child Health Research Program, Department of Obstetrics and Gynecology, Center for Research on Reproduction and Women's Health, University of Pennsylvania, 1353 Biomedical Research Bldg. II/III, 421 Curie Blvd., Philadelphia, PA 19104-6142
    Affiliations
    Maternal and Child Health Research Program, Department of Obstetrics and Gynecology, Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia, PA
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  • Kelsey Breen
    Affiliations
    Maternal and Child Health Research Program, Department of Obstetrics and Gynecology, Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia, PA
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  • Alexander Friedman
    Affiliations
    Maternal and Child Health Research Program, Department of Obstetrics and Gynecology, Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia, PA
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  • Jinghua Chai
    Affiliations
    Maternal and Child Health Research Program, Department of Obstetrics and Gynecology, Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia, PA
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  • Michal A. Elovitz
    Affiliations
    Maternal and Child Health Research Program, Department of Obstetrics and Gynecology, Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia, PA
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      Objective

      The purpose of this study was to investigate whether magnesium sulfate (MgSO4) prevents fetal brain injury in inflammation-associated preterm birth (PTB).

      Study Design

      Using a mouse model of PTB, lipopolysaccharide (LPS) or normal saline solution (NS)–exposed mice were randomized to intraperitoneal treatment with MgSO4 or NS by intrauterine injection. From the 4 treatment groups (NS + NS; LPS + NS; LPS + MgSO4; and NS + MgSO4), fetal brains were collected for quantitative polymerase chain reaction studies and primary neuronal cultures. Messenger RNA expression of cytokines, cell death, and markers of neuronal and glial differentiation were assessed. Immunocytochemistry and confocal microscopy were performed.

      Results

      There was no difference between the LPS + NS and LPS + MgSO4 groups in the expression of proinflammatory cytokines, cell death markers, and markers of prooligodendrocyte and astrocyte development (P > .05 for all). Neuronal cultures from the LPS + NS group demonstrated morphologic changes; this neuronal injury was prevented by MgSO4 (P < .001).

      Conclusion

      Amelioration of neuronal injury in inflammation-associated PTB may be a key mechanism by which MgSO4 prevents cerebral palsy.

      Key words

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      Linked Article

      • Latest research from the 2010 meeting of the Society for Maternal-Fetal Medicine
        American Journal of Obstetrics & GynecologyVol. 202Issue 3
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          This month's issue includes 8 articles that describe research presented at the 2010 meeting of the Society for Maternal-Fetal Medicine (SMFM) in Chicago on February 4-6. From 1256 abstracts submitted, 86 were selected by the Society for oral presentation. Authors of these 86 abstracts were invited to submit their manuscripts through the “Fast-Track” process, in which AJOG reviewers provided rapid reviews in time to allow revision to meet the deadline for inclusion in this issue. These fast-track articles are easily identified by the checkered racing flags on the first page of each as well as next to their titles in the Contents section.
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      • Correction: MARCH 2010 (vol. 202, no. 3, page 292)
        American Journal of Obstetrics & GynecologyVol. 202Issue 6
        • Preview
          In a research article published in March 2010 (Burd I, Breen K, Friedman A, et al. Magnesium sulfate reduces inflammation-associated brain injury in fetal mice. Am J Obstet Gynecol 2010;202:292.e1-9), the first sentence of the Study Design section of the abstract should have read: “In a mouse model of PTB, mice exposed to lipopolysaccharide (LPS) or normal saline (NS) by intrauterine injection were randomized to intraperitoneal treatment with MgSO4 or NS.”
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