Objective
The purpose of this study was to investigate the effect of epigallocatechin gallate
(EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model.
Study Design
ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation
cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were
evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice.
Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected
and evaluated at 4 and 8 weeks after the treatment.
Results
Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of
treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels
(P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors
at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated
group (P < .05).
Conclusion
EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine
leiomyoma cells in vitro and in vivo.
Key words
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Article Info
Publication History
Published online: January 14, 2010
Accepted:
October 28,
2009
Received in revised form:
August 31,
2009
Received:
March 31,
2009
Footnotes
This study was supported by Research Center for Minority Institutes (RCMI) Grant G12 RR03032 and National Institutes of Health/National Institute of Child Health and Human Development 1 R01 HD046228 (A.A.).
Cite this article as: Zhang D, Al-Hendy M, Richard-Davis G, et al. Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. Am J Obstet Gynecol 2010;202:289.e1-9.
Identification
Copyright
© 2010 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
