Effect of experimental diabetes on the development and maintenance of vulvovaginal candidiasis in female rats

Published:March 16, 2009DOI:


      The purpose of this study was to develop an experimental model of diabetes in female rats and verify its influence on vulvovaginal candidiasis.

      Study Design

      The animals were divided into control and diabetic groups. Diabetes was induced with the use of an intravenous solution of alloxan (42 mg/kg bodyweight). One week after confirmation of hyperglycemia, the inoculation of Candida albicans yeast, previously standardized from a vaginal isolate, in concentrations of about 5 × 108, was performed. Infection control was made through vaginal culture, Papanicolaou cytology, and scanning electron microscopy (SCEM).


      The results pointed to different glycemias between the control (74.8 ± 2.6) and experimental groups (543.1 ± 12.1) and a significant bodyweight decrease (227.6 ± 4.77 and 204 ± 6.39, respectively). The positive infection was shown by culture, Papanicolaou test, and SCEM in the experimental group.


      Diabetes mellitus causes hyperglycemia, which was favorable to the vaginal colonization and infection by C albicans.

      Key words

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to American Journal of Obstetrics & Gynecology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Ziarrusta G.B.
        Vulvovaginitis candidiasica.
        Rev Iberoam Micol. 2002; 19: 22-24
        • Holanda A.A.R.
        • Fernandes A.C.S.
        • Bezerra C.M.
        • et al.
        Vulvovaginal candidiasis: symptomatology, risk factors and concomitant anal colonization.
        Rev Bras Ginecol Obstet. 2007; 29: 3-9
        • Fernandes C.E.
        • Machado R.B.
        Etiopathogenic aspects, diagnostic and therapeutics of the vulvovaginal candidiasis.
        Rev Bras Medicin. 1996; 7: 100-104
        • Bohannon N.J.V.
        Treatment of vulvovaginal candidiasis in patients with diabetes.
        Diabetes Care. 1998; 21: 451-456
        • Zdolsek B.
        • Hellberg D.
        • Fröman G.
        • Nilsson S.
        • Mårdh P.A.
        Vaginal microbiological flora and sexually transmitted diseases in women with recurrent or current vulvo vaginal candidiasis.
        Infection. 1995; 23: 81-84
        • Ferraza M.H.S.H.
        • Maluf M.L.F.
        • Consolaro M.E.L.
        • et al.
        Characterization of yeasts isolated from the vagina and their association with vulvovaginal candidiasis in two cities of the South of Brazil.
        Rev Bras Ginecol Obstet. 2005; 27: 58-63
        • Foss N.T.
        • Polon D.P.
        • Takada M.H.
        • Foss-Freitas M.C.
        • Foss M.C.
        Dermatosis in patients with diabetes mellitus.
        Rev Saude Publica. 2005; 39: 677-682
        • Calderon L.
        • Williams R.
        • Martinez M.
        • Clemons K.V.
        • Stevens D.A.
        Genetic susceptibility to vaginal candidiasis.
        Med Mycol. 2003; 41: 143-147
        • Nohmi T.
        • Abe S.
        • Dobashi K.
        • Tansho S.
        • Yamaguchi H.
        Suppression of anti-Candida activity of murine neutrophils by progesterone in vitro: a possible mechanism in pregnant women's vulnerability to vaginal candidiasis.
        Microbiol Immunol. 1995; 39: 405-409
        • Schmid J.
        • Voss E.
        • Soll D.R.
        Computer assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3.
        J Clin Microbiol. 1990; 28: 1236-1243
        • Sobel J.D.
        • Faro S.
        • Force R.W.
        • et al.
        Vulvovaginal candidiasis: epidemiologic, diagnostic, and therapeutic considerations.
        Am J Obstet Gynecol. 1998; 178: 203-211
        • Takahashi M.
        Color atlas of cancer cytology.
        Manole, São Paulo, Brasil1982 (16480)
        • Bibbo M.
        Comprehensive cytopathology.
        W. B. Saunders Co, Philadelphia, PA1997 (101-24)