Objective
This study was undertaken to determine the prevalence of Y-chromosome microdeletions
in couples with recurrent pregnancy loss (RPL) as compared with fertile couples.
Study Design
Forty-three men from couples with recurrent pregnancy loss, and 43 men from couples
with a live birth and no history of miscarriages were recruited from Zekai Tahir Burak
Woman Health, Education and Research Hospital. The DNA was tested for the presence
of 4 sequence tagged sites (STSs) spanning 4 AZF regions: DYS220 (AZFb), DYS235, DYS236,
and DYS237 (AZFd).
Results
Seven (7) of the 43 men (16%) from couples with recurrent pregnancy loss had microdeletions
in 1 or more of the 4 segments studied, whereas none of the fertile men had any microdeletions
(P < .05). Their microdeletions were all found specifically at locus DYS 220 (AZFb).
Conclusion
The prevalence of the Y chromosome microdeletion in AZF region was much higher in
men from couples with recurrent pregnancy loss than men in fertile couples. This study
showed that Y chromosome microdeletion in AZF region may be a possible etiologic factor
of recurrent pregnancy loss.
Key words
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References
- Recurrent miscarriage I: definition and epidemiology.Lancet. 1990; 336: 673-675
- Recurrent miscarriage: etiology, management and prognosis.Hum Reprod Uptake. 2002; 8: 463-481
- Evidence for a strong parenteral effect on human periimplantation embryo development and blastocyst formation.Mol Reprod Dev. 1994; 38: 36-42
- Sperm integrity is critical for normal mitotic division and early embryonic development.Mol Hum Reprod. 1999; 5: 836-844
- Some semen abnormalities may cause infertility by impairing implantation rather than fertilization.Med Hypotheses. 2001; 56: 653-657
- Human male infertility: chromosome anomalies, meiotic disorders, abnormal spermatozoa and recurrent abortion.Hum Reprod Uptake. 2000; 6: 93-105
- Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome long arm.Hum Genet. 1976; 34: 119-124
- Human Y chromosome azospermia factors (AZF) mapped to different subregions in Yq11.Hum Mol Genet. 1996; 5: 933-943
- Defining regions of the Y chromosome responsible for male infertility and identification of fourth AZF region (AZFd) by Y-chromosome microdeletion detection.Mol Reprod Dev. 1999; 53: 27-41
- Y-chromosome microdeletions and recurrent pregnancy loss.Fertil Steril. 2006; 85: 441-445
- Predictive value of abnormal sperm morphology in invitro fertilization.Fertil Steril. 1988; 49: 112-117
- Evidence based management of recurrent miscarriage: optimal diagnostic protocol.International Congress Series. 2004; 1266: 318-327
- Sperm morphology and recurrent abortion.Fertil Steril. 1994; 61: 776-778
- Elevated sperm aneuploidy and apoptosis in patients with unexplained recurrent pregnancy loss.Obstet Gynecol. 2003; 101: 1229-1235
- A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif.Nature. 1990; 346: 240-244
- Y chromosome microdeletions and alterations of spermatogenesis.Endocrine Rev. 2005; 22: 226-229
- Meiotic arrest at the midpachytene stage in a patient with complete azospermiz factor b deletion of the Y chromosome.Fertil Steril. 2006; 85: 494.e5-494.e8
- Meiotic abnormalities in patients bearing complete AZFc deletion of Y chromosome.Hum Reprod. 2007; 22: 1567-1572
- Meiotic anomalies in infertile men with severe spermatogenic defects.Hum Reprod. 2005; 20: 1897-1902
- Molecular diagnosis of Y chromosome microdeletions in Europe: state-of-the-art and quality control.Hum Reprod. 2001; 16: 402-409
Article Info
Publication History
Published online: September 26, 2008
Accepted:
June 3,
2008
Received in revised form:
April 28,
2008
Received:
January 28,
2008
Footnotes
Cite this article as: Karaer A, Karaer K, Ozaksit G, et al. Y-chromosome AZF regions microdeletions and recurrent pregnancy loss. Am J Obstet Gynecol 2008;199:662.e1-662.e5.
Reprints not available from the authors.
Identification
Copyright
© 2008 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
ScienceDirect
Access this article on ScienceDirectLinked Article
- Are Y chromosome microdeletions and recurrent pregnancy loss really associated?American Journal of Obstetrics & GynecologyVol. 201Issue 5
- PreviewWe read with great interest the article by Karaer et al,1 because Y chromosome microdeletions (YMD) and recurrent pregnancy loss (RPL) represent an interesting and poorly studied association. However, we found some weaknesses that need to be clarified: first, they used 4 sequence tagged sites (STS): 1 for the AZFb region (DYS220 [sY129]) and 3 for the AZFd region (DYS235 [sY150], DYS236 [sY152], DYS237 [sY153]). AZFd zone was proposed in 1999, but confirmed evidence shows absence of this fourth zone, and all STS referred to as AZFd used in this study correspond to AZFc zone.
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