Objective
The purpose of this study was to determine the accuracy of the none-invasive prenatal
determination of polymerase chain reaction (PCR)-based fetal RhD genotyping.
Study design
A prospective case series was undertaken on all RhD-negative pregnant women presenting
for genetic counseling in our prenatal diagnosis center from January 2001 until December
2002. Results were compared with serologic RhD typing of the newborns.
Results
Among the 285 pregnant women who participated in the study, fetal RhD status could
be determined for 283 patients. In 2 cases, the RhD-negative phenotype of the mother
was not the result of a complete RHD gene deletion, and therefore, the status of the
fetus could not be determined. Neither false-negative nor false-positive results were
observed.
Conclusion
The present report demonstrates that a reliable fetal RHD genotype determination can
be achieved with 100% accuracy. It is therefore possible to consider that such an
assay could be systematically proposed to all RhD-negative pregnant women in order
to more effectively utilize RhD prophylaxis.
Key words
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Article Info
Publication History
Accepted:
October 28,
2004
Received in revised form:
October 1,
2004
Received:
June 29,
2004
Identification
Copyright
© 2005 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
