Abstract
Objective: We report the prenatal detection by fluorescence in situ hybridization analysis of
a male fetus with trisomy 18. Study Design: Total nucleated cells recovered from 7 mL of maternal peripheral blood by means of
double-density gradient centrifugation were cultured for 3 days in a devised medium.
Results: Fetal cells with X- and Y-specific signals were detected in all the established cultures,
but the yield and purity were higher in the culture from the 1077 Ficoll layer. Cumulatively,
84 fetal cells were recorded by analysis of 5640 cells. The hematopoietic lineages
involved in the production of the fetal cells in culture were not assessed. For the
cultures established with the 1119 Ficoll layer, the involvement of progenitors or
precursors of the erythroid lineage was assumed because postculture sorting was directed
toward cells expressing the erythropoietin receptor. Conclusion: We conclude that culturing total nucleated cells from maternal blood is a new procedure
that could prove valuable in the detection of the main fetal aneuploidies affecting
pregnant populations. (Am J Obstet Gynecol 2000;183:222-5.)
Keywords
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Article info
Publication history
Accepted:
January 13,
2000
Received in revised form:
September 22,
1999
Received:
June 14,
1999
Footnotes
☆Reprint requests: Domenico Valerio, Istituto di Ricerche Genetiche, Viale della Costituzione Is. F3, Centro Direzionale, Napoli, Italy.
Identification
Copyright
© 2000 Mosby, Inc. Published by Elsevier Inc. All rights reserved.
