An increase in expression of the lipocalin 24p3 is found in mouse uterus coincident with birth

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      OBJECTIVE: To provide novel insights into the molecular events associated with parturition, a differential screen was made of a mouse uterine complementary deoxyribonucleic acid library to identify selectively expressed genes in late pregnancy.
      STUDY DESIGN: A differential hybridization was used to screen a mouse complementary deoxyribonucleic acid library prepared from late pregnancy uterus. A 1 kb clone was isolated that was subsequently identified as 24p3, a member of the lipocalin family. By use of radiolabeled complementary deoxyribonucleic acid probes prepared from this clone Northern hybridization were conducted against total ribonucleic acid purified from mouse uterus collected during late pregnancy and the first week after birth and a variety of other mouse tissues to determine whether this gene is selectively expressed in the uterus coincident with parturition.
      RESULTS: Low levels of message for the 24p3 gene could be detected in uterine ribonucleic acid, but there was a massive increase in the level of message for this gene on the days surrounding birth. Northern hybridizations conducted against additional tissues collected from both pregnant and nonpregnant mice did not detect message to a similar degree as found in the uterus.
      CONCLUSIONS: The uterus constitutes a major site of expression of this gene, particularly near birth. The expression of this gene coincident with birth suggests a potential physiologic role of the neutrophil with the induction of labor.


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        • Ali S
        • Clark A
        Characterization of the gene encoding ovine beta-lactoglobulin.
        J Mol Biol. 1988; 199: 415-426
        • Pervaiz S
        • Brew K
        Homology of beta-lactoglobulin, serum retinol-binding protein and protein HC.
        Science. 1985; 228: 335-337
        • Pervaiz S
        • Brew K
        Homology and structure-function correlations between alpha 1-acid glycoprotein and serum retinol-binding protein and its relatives.
        FASEB J. 1987; 1: 209-214
        • Godovac-Zimmerman J
        The structural motif of beta-lactoglobulin and retinol binding protein: a basic framework for binding and transport of small hydrophobic molecules?.
        Trends Biochem Sci. 1988; 13: 64-66
        • Akerstrom B
        • Logberg L
        An intriguing member of the lipocalin protein family: alpha 1 microglobulin.
        Trends Biochem Sci. 1990; 15: 240-243
        • Bocskei Z
        • Groom C
        • Flower D
        • et al.
        Pheromone binding to two rodent urinary proteins revealed by x-ray crystallography.
        Nature. 1992; 360: 186-188
        • Cowan S
        • Newcomer M
        • Jones T
        Crystallographic refinement of human serum retinol binding protein at 2A resolution.
        Proteins. 1990; 8: 44-61
        • Hraba-Renevey S
        • Turler H
        • Kress M
        • Salomon C
        • Weil R
        SV-40 induced expression of mouse gene 24p3 involves a post-transcriptional mechanism.
        Oncogene. 1989; 4: 601-608
        • Chan Y
        • Paz V
        • Wool I
        The primary structure of rat alpha 2u globulin-related protein.
        Nucleic Acids Res. 1989; 17: 6401
        • Kjeldsen L
        • Johnsen A
        • Sengelov H
        • Borregaard N
        Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase.
        J Biol Chem. 1993; 268: 10425-10432
        • Triebel S
        • Blaser J
        • Reinke H
        • Tschesche H
        A 25 kDa alpha 2 microglobulin related protein is a component of the 125 kDa form of human gelatinase.
        FEBS Lett. 1992; 314: 386-388
        • Peitsch M
        • Boguski M
        The first lipocalin with enzymatic activity.
        Trends Biochem Sci. 1991; 16: 363
        • Flower D
        • North A
        • Attwood T
        Mouse oncogene protein 24p3 is a member of the lipocalin protein family.
        Biochem Biophys Res Commun. 1991; 180: 69-74
        • Kjeldsen L
        • Bainton D
        • Sengelov H
        • Borregaard N
        Identification of neutrophil gelatinase-associated lipocalin as a novel maurix protein of specific granules in human neutrophils.
        Blood. 1994; 83: 799-807
        • Sengelov H
        • Boulay F
        • Kjeldsen L
        • Borregaard N
        Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils.
        Biochem J. 1994; 299: 473-479
        • Kasik J
        • Rice E
        Kidney androgen-regulated gene is expressed in the uterus during the pregnancy.
        Mol Cell Endocrinol. 1993; 90: 239-242
        • Sargent T
        Isolation of differentially expressed genes.
        Methos Enzymol. 1987; 152: 423-432
        • Chomczynski P
        • Sacchi N
        Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction.
        Anal Biochem. 1987; 162: 156-159
        • Sambrook J
        • Fritsch E
        • Maniatis T
        4th ed. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York1989
        • Gish W
        • States D
        Identification of protein coding regions by database similarity search.
        Nat Genet. 1993; 3: 266-272
        • Marbaix F
        • Donnez J
        • Courtoy P
        • Eeckhout Y
        Progesterone regulates the activity of collagenase and related gelatinase A and B in human endometrial explants.
        in: 4th ed. Proc Natl Acad Sci U S A. 89. 1992: 11789-11793
        • Casey M
        • MacDonald P
        The onset of labor: cellular and integrative mechanisms. Perinatology Press, Ithaca, New York1988: 141-156
        • Casey M
        • Cox S
        • Word R
        • MacDonald P
        Cytokines and infection-induced preterm labor.
        Reprod Fertil Dev. 1990; 2: 499-509
        • Hillier S
        • Witkin S
        • Krohn M
        • Watts D
        • Kiviat N
        • Eschenbach D
        The relationship of amniotic fluid cytokines and preterm delivery, amniotic fluid infection, histologic chorioamnionitis and chorioamnion infection.
        Obstet Gynecol. 1993; 81: 941-948
        • Ghosh P
        • Steger R
        • Bartke A
        Possible involvement of hypothalamic monoamines in mediating the action of alpha-2u-globulin on the pituitary-testicular axis in rats.
        Neuroendocrinology. 1991; 53: 7-11