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Mitogenic effect of basic fibroblast growth factor and estradiol on cultured human myometrial and leiomyoma cells

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      Abstract

      Objective: We compared the mitogenic effect of basic fibroblast growth factor with and without estradiol on myometrial and leiomyometrial cells.
      Study Design: The mitogenic effect of basic fibroblast growth factor on myometrial cells was measured by thymidine incorporation and cell count. The mitogenic effect of basic fibroblast growth factor with and without estradiol as measured by thymidine incorporation was compared between myometrial and leiomyometrial cells.
      Results: Both human myometrial and leiomyometrial cells showed significant (p = 0.004 and p = 0.001, respectively), dose-dependent incorporation of thymidine in response to basic fibroblast growth factor. Leiomyometrial cells showed significantly (p = 0.04) less thymidine incorporation compared with matched normal myometrial cells. The addition of estradiol with basic fibroblast growth factor did not result in a further increase in thymidine incorporation.
      Conclusions: Both myometrial and leiomyometrial cells respond to basic fibroblast growth factor with increased thymidine incorporation; however leiomyometrial cells are less responsive than are matched normal myometrial cells. The addition of estradiol is not synergistic with basic fibroblast growth factor.

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      References

        • Buttram VC
        • Reiter RC
        Uterine leiomyomata: etiology, symptomatology, and management.
        Fertil Steril. 1981; 36: 433-445
        • West CP
        • Lumsden MA
        • Lawson S
        • Williamson J
        • Baird DT
        Shrinkage of uterine fibroids during therapy with goserelin (Zoladex): a luteinizing hormone-releasing hormone agonist administered as a monthly subcutaneous depot.
        Fertil Steril. 1987; 48: 45-51
        • Otsuka H
        • Shinohara M
        • Kashimura M
        • Yoshida K
        • Okamura Y
        A comparative study of the estrogen receptor ratio in myometrium and uterine leiomyomas.
        Int J Gynecol Obstet. 1989; 29: 189-194
        • Tommola P
        • Pekonen F
        • Ruranen EM
        Binding of epidermal growth factor and insulin-like growth factor I in human myometrium and leiomyomata.
        Obstet Gynecol. 1989; 74: 658-662
        • Cordon-Cardo C
        • Vlodavsky I
        • Haimovitz-Friedman A
        • Hicklin D
        • Fuks Z
        Expression of basic fibroblast growth factor in normal human tissues.
        Lab Invest. 1990; 63: 832-840
        • Rifkin DB
        • Moscatelli D
        Recent developments in the cell biology of basic fibroblast growth factor.
        J Cell Biol. 1989; 109: 1-6
        • Takahashi JA
        • Mori H
        • Fukumoto M
        • et al.
        Gene expression of fibroblast growth factors in human gliomas and meningiomas: demonstration of cellular source of basic fibroblast growth factor mRNA and peptide in tumor tissues.
        in: 5th ed. Proc Natl Acad Sci U S A. 87. 1990: 5710-5714
        • Presta M
        Sex hormones modulate the synthesis of basic fibroblast growth factor in human endometrial adenocarcinoma cells: implications for the neovacularization of normal and neoplastic endometrium.
        J Cell Physiol. 1988; 137: 593-597
        • Carlone DL
        • Rider V
        Embryonic modulation of basic fibroblast growth factor in the rat uterus.
        Biol Reprod. 1993; 49: 653-665
        • Beck CA
        • Garner CW
        Stimulation of DNA synthesis in rat uterine cells by growth factors and uterine extracts.
        Mol Cell Endocrinol. 1992; 84: 109-118
        • Paris S
        • Pouyssegus J
        Mitogenic effects of fibroblast growth factors in cultured fibroblasts.
        Ann N Y Acad Sci. 1991; 628: 139-148
        • Resnick JL
        • Bixler LS
        • Cheng L
        • Donovan PJ
        Long-term proliferation of mouse primordial germ cells in culture.
        Nature. 1992; 359: 550-551
        • Fayed YM
        • Tsibris JCM
        • Langenberg PW
        • Robertson AL
        Human uterine leimyoma cells: binding and growth responses to epidermal growth factor, platelet-derived growth factor, and insulin.
        Lab Invest. 1989; 60: 30-37
        • Cramer SF
        • Robertson AL
        • Ziats NP
        • Pearson OH
        Growth potential of human uterine leiomyomas: some in vitro observations and their implications.
        Obstet Gynecol. 1985; 66: 36-41
        • Gloudemans T
        • Prinsen I
        • Van Unnik JAM
        • Lips CJM
        • otter WD
        • Sussenbach JS
        Insulin-like growth factor gene expression in human smooth muscle tumors.
        Cancer Res. 1990; 50: 6689-6695
        • Speir V
        • Jenkins S
        • White MC
        Growth factor regulation of 17β-gtdrixtsteroid dehydrogenase activity in a human ovarian cell line: modulation by 17β-estradiol.
        Anticancer Res. 1993; 13: 1399-1404
        • Bacher M
        • Rausch U
        • Goebel HW
        • Polzar B
        • Mannherz HG
        • Aumuller G
        II: Stromal and epithelial cells from rat ventral prostate during androgen deprivation and estrogen treatment—regulation of transcription.
        Exp Clin Endocrinol. 1993; 101: 78-86
        • Krosl J
        • Breskvar K
        • Hudnik-Plevnik T
        Prolonged cultivation of rat uterine cells with preserved estrogen responsiveness.
        J Steroid Biochem. 1989; 33: 189-194
        • Lingham RB
        • Stancel GM
        • Loose-Mitchell DS
        Estrogen regulation of epidermal growth factor receptor messenger ribonucleic acid.
        Mol Endocrinol. 1988; 2: 230-235
        • Murphy LJ
        • Murphy LC
        • Friesen HG
        Estrogen induces insulin-like growth factor-1 expression in the rat uterus.
        Mol Endocrinol. 1987; 1: 445-450
        • Sadowsky Y
        • Riemer RK
        • Roberts JM
        The concentration of estrogen receptors in rabbit uterine myocytes decreases in culture.
        Am J Obstet Gynecol. 1992; 167: 1631-1635
        • Lumsden MA
        • West CP
        • Bramley T
        • Rumgay L
        • Baird DT
        The binding of epidermal growth factor to the human uterus and leiomyomata in women rendered hypoestrogenic by continuous administration of an LHRH agonist.
        Br J Obstet Gynaecol. 1988; 95: 1299-1304
        • Rein MS
        • Friedman AJ
        • Pandian MR
        • Heffner LJ
        The secretion of insulin-like growth factors I and II by explant culture of fibroids and myometrium from women treated with gonadotropin-releasing hormone agonist leuprolide acetate.
        Fertil Steril. 1990; 46: 1554-1558
        • Giudice LC
        • Irwin JC
        • Dsupin BA
        • et al.
        Insulin-like growth factor (IGF), IGF binding protein (IGFBP), and IGF receptor gene expression and IGFBP sysnthesis in human uterine leiomyomata.
        Hum Reprod. 1993; 8: 1796-1806
        • Hu J
        • Surti U
        Subgroups of uterine leiomyomas based on cyogenetic analysis.
        Hum Pathol. 1991; 22: 1009-1016