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The effect of benzo(a)pyrene on murine ovarian and corpora lutea volumes

  • Author Footnotes
    a From the Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences
    ,
    Author Footnotes
    b the Division of Developmental Toxicology, National Center for Toxicological Research
    Michael M. Miller
    Correspondence
    Reprint requests: Michael M. Miller, MD, Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, 4301 W. Markham, Slot 518, Little Rock, AR 72205.
    Footnotes
    a From the Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences
    b the Division of Developmental Toxicology, National Center for Toxicological Research
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  • Author Footnotes
    b the Division of Developmental Toxicology, National Center for Toxicological Research
    ,
    Author Footnotes
    c the Integrated Toxicology Program, Duke University Medical Center
    David R. Plowchalk
    Footnotes
    b the Division of Developmental Toxicology, National Center for Toxicological Research
    c the Integrated Toxicology Program, Duke University Medical Center
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  • Author Footnotes
    a From the Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences
    ,
    Author Footnotes
    b the Division of Developmental Toxicology, National Center for Toxicological Research
    Glenn A. Weitzman
    Footnotes
    a From the Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences
    b the Division of Developmental Toxicology, National Center for Toxicological Research
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  • Author Footnotes
    d the Department of Obstetrics and Gynecology, University of Kentucky
    Steve N. London
    Footnotes
    d the Department of Obstetrics and Gynecology, University of Kentucky
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  • Author Footnotes
    b the Division of Developmental Toxicology, National Center for Toxicological Research
    ,
    Author Footnotes
    e the Graduate School of Public Health, University of Pittsburgh.
    Donald R. Mattison
    Footnotes
    b the Division of Developmental Toxicology, National Center for Toxicological Research
    e the Graduate School of Public Health, University of Pittsburgh.
    Search for articles by this author
  • Author Footnotes
    a From the Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences
    b the Division of Developmental Toxicology, National Center for Toxicological Research
    c the Integrated Toxicology Program, Duke University Medical Center
    d the Department of Obstetrics and Gynecology, University of Kentucky
    e the Graduate School of Public Health, University of Pittsburgh.
      This paper is only available as a PDF. To read, Please Download here.
      Objective: Women who smoke have impaired fertility and experience menopause at an earlier age. This experiment determined the effect of benzo(a)pyrene, a polycyclic aromatic hydrocarbon contained in cigarette smoke, on murine ovarian volume, total corpora lutea volume, individual corpora lutea volumes, and corpora lutea numbers.
      Study Design: C57BL/6N mice were treated with intraperitoneal injections of 0 to 500 mg/kg benzo(a)pyrene in corn oil. The 20 mice at each dose were divided into four groups of five each and were killed at 1, 2, 3, or 4 weeks after treatment. Ovaries were serially sectioned and analyzed morphometrically.
      Results: Benzo(a)pyrene produced a dose- and time-dependent decrease in ovarian volume, total corpora lutea volume, and number of corpora lutea per ovary. This effect was transitory at low doses with complete recovery of corpora lutea by 4 weeks. Compensatory hypertrophy of the individual corpora lutea occurred during the recovery phase. Ovarian function did not return in animals treated with the two highest doses.
      Conclusion: Benzo(a)pyrene is a murine ovarian toxicant that inhibits corpus luteum formation in a dose- and time-dependent fashion.

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