Advertisement

Ultrastructure of the microvasculature in the human endometrium throughout the normal menstrual cycle

  • Author Footnotes
    a From the Department of Obstetrics and Gynecology, University of Kansas School of Medicine-Wichita, HCA-Wesley Medical Center
    Daniel K. Roberts
    Footnotes
    a From the Department of Obstetrics and Gynecology, University of Kansas School of Medicine-Wichita, HCA-Wesley Medical Center
    Search for articles by this author
  • Author Footnotes
    b Departments of Obstetrics and Gynecology and Pathology, University of Arkansas for Medical Sciences.
    Tim H. Parmley
    Footnotes
    b Departments of Obstetrics and Gynecology and Pathology, University of Arkansas for Medical Sciences.
    Search for articles by this author
  • Author Footnotes
    a From the Department of Obstetrics and Gynecology, University of Kansas School of Medicine-Wichita, HCA-Wesley Medical Center
    Nola J. Walker
    Correspondence
    Reprint requests: Nola J. Walker, EMSA, Electron Microscopy Laboratory, Department of Obstetrics and Gynecology, HCA-Wesley Medical Center, 550 N. Hillside, Wichita, KS 67214.
    Footnotes
    a From the Department of Obstetrics and Gynecology, University of Kansas School of Medicine-Wichita, HCA-Wesley Medical Center
    Search for articles by this author
  • Author Footnotes
    a From the Department of Obstetrics and Gynecology, University of Kansas School of Medicine-Wichita, HCA-Wesley Medical Center
    Douglas V. Horbelt
    Footnotes
    a From the Department of Obstetrics and Gynecology, University of Kansas School of Medicine-Wichita, HCA-Wesley Medical Center
    Search for articles by this author
  • Author Footnotes
    a From the Department of Obstetrics and Gynecology, University of Kansas School of Medicine-Wichita, HCA-Wesley Medical Center
    b Departments of Obstetrics and Gynecology and Pathology, University of Arkansas for Medical Sciences.
      This paper is only available as a PDF. To read, Please Download here.
      In the early proliferative phase endometrial capillary basal lamina is loosely formed and discontinuous. It becomes more distinct in the late proliferative phase and develops whorled extensions that include pericytes in the early secretory to midsecretory phase. Throughout the cycle both endothelial cells and pericytes possess cytoplasmic processes that extend through discontinuities in the basal lamina and make contact with each other. These contacts may be simple or complex. They become more elaborate as the cycle progresses to the midsecretory phase. Endothelial cells show progressive but heterogeneous differentiation into the midsecretory phase, with individual cell hypertrophy. In the late-secretory phase the endometrial stroma and the basal lamina are characterized by widespread degeneration, and the cell-to-cell contacts sharply decrease. However, endothelial cells remain relatively intact.

      Key words

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to American Journal of Obstetrics & Gynecology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Roberts DK
        • Walker NJ
        • Lavia LA
        Ultrastructural evidence of stromal/epithelial interactions in the human endometrial cycle.
        Am J Obstet Gynecol. 1989; 158: 854-861
        • Parmley TH
        • Roberts DK
        • Walker NJ
        • Horbell DV
        Intercellular contacts between stromal cells in the normal human endometrium throughout the menstrual cycle.
        Hum Pathol. 1990; 21: 1063-1066
        • Simionescu N
        • Simionescu M
        The cardiovascular system.
        in: Weiss L Histology, cell and tissue biology. ed 5. Elsevier Biochemical, New York1983
        • Larson DM
        • Carson MP
        • Haudenschild CC
        Gapjunctions in endothelial cells and pericytes.
        in: Tsuchiya M Asano M Misima Y Odad M Microcirculation-an update, part I and II. Elsevier Science, New York1987: 101-104
        • D'Amore PA
        • Orlidge A
        • Herman IM
        Growth control in the retinal microvasculature.
        in: Osborne N Chader GJ Progress in retinal research. Pergamon, Oxford1988: 233-258
        • Antonelli-Orlidge A
        • Saunders KB
        • Smith SR
        • D'Amore PA
        An activated form of transforming growth factor beta is produced by cocultures of endothelial cells and pericytes.
        Proc Natl Acad Sci USA. 1989; 86: 4544-4548
        • Wynn RM
        The human endometrium: cyclic and gestational changes.
        in: Wynn RM Jollie WP Biology of the uterus. Plenum, New York1989: 289-331
        • Denker HE
        • Enders AC
        • Schlafke S
        Bizarre hypertrophy of vascular endothelial cells in rhesus monkey endometrium: experimental induction and electron microscopical characteristics.
        Verh Anat Ges. 1985; 79: 545-548
        • Enders AC
        • Welsh AO
        • Schlafke S
        Implantation in the rhesus monkey: endometrial responses.
        Am J Anat. 1985; 173: 147-169
        • Enders AC
        • Schlafke S
        Implantation in nonhuman primates and in the human.
        in: Dukelow WR Erwin J Comparative primate biology, reproduction and development. vol 3. Alan R Liss, New York1986: 291-310
        • Tarara R
        • Enders AC
        • Hendrickx AG
        • et al.
        Early implantation and embryonic development of the baboon: stages 5, 6, and 7.
        Anat Embryol. 1987; 176: 267-275
        • Noyes RW
        • Hertig AT
        • Rock J
        Dating the endometrial biopsy.
        Fertil Steril. 1950; 1: 3-26
        • Watson ML
        Staining of tissue sections for electron microscopy with heavy metals.
        J Biophys Biochem Cytol. 1958; 4: 475-478
        • Millonig GJ
        Advantages of phosphate buffer of Os04 solutions in fixation.
        J Appl Physiol. 1961; 32: 1637-1641
        • Reynolds ES
        The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
        J Cell Biol. 1963; 17: 208-212
        • Spurlock BO
        • Skinner MS
        • Kattine AA
        A simple rapid method for staining epoxy-embedded specimens for light microscopy with the Polychromate stain paragon-1301.
        Am J Clin Pathol. 1966; 46: 252-258
        • Fawcett DW
        Specializations of the free surface.
        in: Meier A The cell. ed 2. WB Saunders, Philadelphia1987: 104-105
        • Kalimi GH
        • Lo CW
        Gap junctional communication in the extraembryonic tissues of the gastrulating mouse embryo.
        J Cell Biol. 1989; 109: 3015-3026
        • Slack JMW
        Peptide regulatory factors in embryonic development.
        Lancet. 1989; 1: 1312-1315
        • Wakui S
        • Furusato M
        • Tanaka M
        • Allsbrook Jr, WC
        • Kano Y
        • Ushigome S
        Endothelium and pericyte interdigitation pathway for epidermal growth factor?.
        Microvas Res. 1990; 40: 285-291
        • Bigsby RM
        • Cunha GR
        Estrogen stimulation of deoxyribonucleic acid synthesis in uterine epithelial cells which lack estrogen receptors.
        Endocrinology. 1986; 119: 390-396
        • Cooke PS
        • Uchima F-DA
        • Fujii DK
        • Bern HA
        • Cunha GR
        Restoration of normal morphology and estrogen responsiveness in cultured vaginal and uterine epithelia transplanted with stroma.
        Proc Natl Acad Sei USA. 1986; 83: 2109-2113
        • Cooke PS
        • Fujii DK
        • Cunha GR
        Vaginal and uterine stroma maintain their inductive properties following primary culture.
        In Vitro Cell Dev Biol. 1987; 23: 159-166
        • Ramsey EM
        Vascular anatomy.
        in: Wynn RM Biology of the uterus. ed 1. Plenum Press, New York1977: 59-76
        • Wilborn WH
        • Flowers Jr, CE
        Cellular mechanisms for endometrial conservation during menstrual bleeding.
        Semin Reprod Endocrinol. 1984; 2: 307-341
        • Fajardo LF
        Special report: the complexity of endothelial cells.
        Am J Clin Pathol. 1989; 92: 241-250
        • Wagner DD
        • Marder VJ
        Biosynthesis of von Willebrand protein by human endothelial cells. Identification of a large precursor polypeptide chain.
        J Biol Chem. 1983; 258: 2065-2067
        • Weibel ER
        • Palade GE
        New cytoplasmic components in arterial endothelia.
        J Cell Biol. 1964; 23: 101-112
        • Sengel A
        • Stoebner P
        Golgi origins of tubular inclusions in endothelial cells.
        J Cell Biol. 1970; 44: 223-226
        • Wagner DD
        Storage and secretion of von Willebrand factor.
        in: Zimmerman TS Ruggeri ZM Hematology, coagulation and bleeding disorders: the role of factor VIII and von Willebrand factor. 9. Marcel Dekker, New York1989: 161-180