Human endometrial prostaglandin E2 binding sites and their profiles during the menstrual cycle and in pathologic states

      This paper is only available as a PDF. To read, Please Download here.


      Endometrial tissue from uteri of 35 nonpregnant, premenopausal women was assayed for prostaglandin E2 and F binding site content as a function of the phase of the menstrual cycle and the pathologic state. For all specimens, tritium-labeled prostaglandin F binding was very low (<8 fmol/mg of protein) or undetectable regardless of the phase of the menstrual cycle or pathologic state or in the presence or absence of 10 μmol/L of indomethacin, a prostaglandin synthetase inhibitor. However, tritium-labeled prostaglandin E2 binding was detected in every specimen and was independent of the presence or absence of indomethacin. Binding of tritium-labeled prostaglandin E2, as determined by Scatchard analyses, was biphasic (dissociation constant ∼ 1 nmol/L; dissociation constant for low-affinity sites ∼ 10 nmol/L) for both proliferative and secretory endometrial tissue. However, the total number of prostaglandin E2 binding sites, determined from Scatchard or single-point analyses, was significantly higher (p < 0.01) in proliferative endometrium compared to secretory endometrium. In addition, for endometrium from the proliferative phase of the menstrual cycle, the diagnosis of abnormal uterine bleeding was associated with higher (p < 0.01) tritium-labeled prostaglandin E2 binding than diagnosis of dysmenorrhea, stress urinary incontinence and uterine prolapse, or pelvic inflammatory disease. Endometrial specimens with the last four diagnoses did not differ significantly (p > 0.1) from each other.


      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to American Journal of Obstetrics & Gynecology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Kurzrok R
        • Leib CC
        Biochemical studies of human semen. II. The action of semen on the human uterus.
        in: Proc Soc Exp Biol Med. 28. 1930: 268
        • Eliasson R
        • Posse N
        The effect of prostaglandins on the nonpregnant human uterus in vivo.
        Acta Obstet Gynecol Scand. 1960; 39: 112
        • Bygdeman M
        The effect of different prostaglandins on human myometrium in vitro.
        Acta Physiol Scand. 1964; 63 (suppl): 1
        • Hofmann GE
        • Rao ChV
        • Barrows GH
        • Sanfilippo JS
        Topography of human uterine prostaglandin E and F receptors and their profiles during pathological states.
        J Clin Endocrinol Metab. 1983; 57: 360
        • Hofmann GE
        • Rao ChV
        • Barrows GH
        • Sanfilippo JS
        Prostaglandin E and F receptors in human uterine leiomyomas.
        J Clin Endocrinol Metab. 1984; 58: 454
        • Wakeling AE
        • Wyngarden LJ
        Prostaglandin receptors in the human, monkey and hamster uterus.
        Endocrinology. 1974; 95: 55
        • Kimball FA
        • Kirton KT
        • Spilman CH
        • Wyngarden LJ
        Prostaglandin E1 specific binding in human myometrium.
        Biol Reprod. 1975; 13: 482
        • Rao ChV
        Differential effects of detergents and dimethylsulfoxide on membrane prostaglandin E1 and F receptors.
        Life Sci. 1977; 20: 2013
        • Lowry OH
        • Rosebrough NJ
        • Farr AL
        • Randall RJ
        Protein measurement with the Folin phenol reagent.
        J Biol Chem. 1951; 193: 265
        • Rao ChV
        Characterization of prostaglandin receptors in the bovine corpus luteum cell membranes.
        J Biol Chem. 1974; 249: 7203
        • Rao ChV
        Receptors for various prostaglandins.
        in: Curtis-Prior PB Handbook of biology and chemistry of prostaglandins and related metabolites and polyunsaturated fatty acids. Churchill Livingstone, Edinburgh1984
        • Scatchard G
        The attraction of proteins for small molecules and ions.
        Ann NY Acad Sci. 1949; 51: 660
        • Rao ChV
        Lack of negative cooperativity among binding sites for prostaglandin F in bovine corpus luteum cell membranes.
        Life Sci. 1976; 18: 499
        • Munson PJ
        • Rodbard D
        Ligand: a versatile computerized approach for characterization of ligand binding systems.
        Anal Biochem. 1980; 197: 220
        • Steel RG
        • Torrie JH
        Principles and procedures of statistics.
        McGraw-Hill, New York1960
        • Downie J
        • Poyser NL
        • Wunderlich M
        Levels of prostaglandins in human endometrium during the normal menstrual cycle.
        J Physiol. 1974; 236: 465
        • Smith SK
        • Abel MH
        • Kelly RW
        • Baird DT
        The synthesis of prostaglandins from persistent proliferative endometrium.
        J Clin Endocrinol Metab. 1982; 55: 284
        • Lundstrom V
        • Green K
        Endogenous levels of prostaglandin F and its main metabolites in plasma and endometrium of normal and dysmenorrheic women.
        Am J Obstet Gynecol. 1978; 130: 640
        • Rao ChV
        Prostaglandin F receptors in bovine corpora lutea throughout pregnancy.
        Biol Reprod. 1976; 15: 134
        • Lau IF
        • Saksena SK
        • Chang MC
        Pregnancy blockade by indomethacin, an inhibitor of prostaglandin synthesis: its reversal by prostaglandins and progesterone in mice.
        Prostaglandins. 1973; 4: 795
        • Castracane VD
        • Saksena SK
        • Shaikh AA
        Effect of IUD's prostaglandins and indomethacin on decidual cell reaction in the rat.
        Prostaglandins. 1974; 6: 387
        • Evans CA
        • Kennedy TG
        The importance of prostaglandin synthesis for the initiation of blastocyst implantation in the hamster.
        J Reprod Fertil. 1978; 54: 255
        • Hoffman LH
        • DiPietro DL
        • McKenna TJ
        Effects of indomethacin on uterine capillary permeability and blastocyst development in rabbits.
        Prostaglandins. 1978; 15: 823