Advertisement

The effect of a prostaglandin synthetase inhibitor on the hormonal profile and the endometrium in women

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      A prostaglandin synthetase inhibitor, naproxen, was given continuously throughout the menstrual cycle at a dose of 250 mg twice daily to 10 healthy fertile women (group 1) and at a dose of 1000 mg per day to eight women in the secretory phase (group 2). Blood samples were withdrawn three times a week during a control cycle and during the treatment cycle. Luteinizing hormone, follicle-stimulating hormone, prolactin, estradiol, and progesterone were analyzed. Endometrial biopsies were taken in the secretory phase of the control cycle and in the treatment cycle of group 1 and on the first day of menstruation of group 2. Naproxen treatment did not suppress ovulation in any cycle and did not affect the corpus luteum function either in group 1 or in group 2. In only one of the endometrial samples taken in the secretory phase (group 1) was the density of the lysosomes increased in the treatment cycle compared to the control cycle. The specimen taken during the early menstrual period (group 2) showed an increase in glandular epithelium and height following naproxen treatment. Furthermore, a significant increase in the number of plasmolemmal vesicles per square micrometer was observed in the capillary endothelial cells after the administration of 1000 mg of naproxen per day. This suggests that the transcellular exchange of water-soluble molecules in the endothelial cells was more active after the administration of a prostaglandin synthetase inhibitor than in the control group. In spite of the significant morphologic changes observed in the naproxen-treated material the onset of menstrual bleeding could not be prevented. The mechanism of the onset of menstruation needs to be further investigated.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to American Journal of Obstetrics & Gynecology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • LeMaire W
        • Yang NST
        • Behrman HR
        • Marsh JM
        Preovulatory changes in the concentration of prostaglandins in rabbit graafian follicles.
        Prostaglandins. 1973; 3: 36
        • Armstrong DT
        • Grimmich DL
        Blockade of spontaneous and LH induced ovulation in rats by indomethacin on inhibition of prostaglandin biosynthesis.
        Prostaglandins. 1972; 1: 21
        • Hamberger L
        • Nilsson L
        • Dennefors B
        • Khan I
        • Sjögren A
        Cyclic AMP formation of isolated human corpora lutea in response to HCG-interference by PGF.
        Prostaglandins. 1979; 17: 615
        • Hamberger L
        • Källfelt B
        • Forshell S
        • Dukes M
        A luteolytic effect of a prostaglandin F analogue in non-pregnant women.
        Contraception. 1980; 22: 383
        • Chaudhuri G
        • Elder MG
        Lack of evidence for inhibition of ovulation by aspirin in women.
        Prostaglandins. 1976; 11: 727
        • Landgren BM
        • Undén AL
        • Diczfalusy E
        Hormonal profile of the cycle in 68 normally menstruating women.
        Acta Endocrinol. 1980; 94: 89
        • Smith RE
        • Henzl MR
        Role of mucopolysaccharides and lysosomal hydrolases in endometrial regression following withdrawal of estradiol and chlormadinone acetate. I. Epithelium and stroma.
        Endocrinology. 1969; 85: 50
        • Luft JH
        Improvements in epoxy resin embedding methods.
        J Biophys Biochem Cytol. 1961; 9: 409
        • Watson ML
        Staining of tissue sections for electron microscopy with heavy metals.
        J Biophys Biochem Cytol. 1958; 4: 457
        • Reynolds ES
        The use of lead citrate at high pH as an electron opaque stain in electron microscopy.
        J Cell Biol. 1963; 17: 208
        • Johannisson E
        • Parker RA
        • Landgren BM
        • Diczfalusy E
        Morphometric analysis of the human endometrium in relation to peripheral hormone levels. I. A re-assessment of the histological dating of endometrial biopsies.
        Fertil Steril. 1982; 38: 569
        • Toppozada M
        • El-Abd M
        • El-Sokkary H
        • Khali T
        • Galal R
        • El-Rhaman HA
        Effect of a prostaglandin inhibitor on human ovulation.
        Singapore J Obstet Gynecol. 1979; 10: 42
        • Wallach EE
        • de la Cruz A
        • Hunt J
        • Wright KH
        • Stevens VC
        The effect of indomethacin on HMG-HCG induced ovulation in the Rhesus monkey.
        Prostaglandins. 1975; 9: 645
        • Lundström V
        Treatment of primary dysmenorrhoea with prostaglandin synthetase inhibitors.
        Acta Obstet Gynecol Scand. 1978; 57: 421
      1. Lenton EA, Landgren BM, Sexton L. Normal variation in the length of the luteal phase of the menstrual cycle: identification of the short luteal phase Br J Obstet Gynaecol (in press).

        • Weissman G
        • Goldstein I
        • Hoffstein S
        Prostaglandins and the modulation by cyclic nucleotides of lysosomal enzyme release.
        in: Samuelsson B Paoletti R Advances in prostaglandin and thromboxane research. Raven Press, New York1976: 803
        • Briggs M
        Lysosomal enzyme activation by steroid hormones in vivo.
        Biochemistry. 1973; 4: 341
        • Szego CM
        The lysosome as a mediator of hormone action.
        Recent Prog Horm Res. 1974; 30: 171
        • Majno G
        • Joris I
        Endothelium 1977: a review.
        Adv Exp Med Biol. 1978; 104: 225
        • Smith JB
        • Willis AL
        Aspirin selectively inhibits prostaglandin production in human platelets.
        Nature. 1971; 231: 235
        • Wolfe SM
        • Schulman NR
        Adenyl cyclase activity in human platelets.
        Biochem Biophys Res Commun. 1969; 35: 265