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A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone

  • Judith L. Vaitukaitis
    Correspondence
    Reprint requests: Dr. J. Vaitukaitis, Dept. of Health, Education, and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland, 20014
    Affiliations
    Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Besthesda, Maryland, USA
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  • Glenn D. Braunstein
    Affiliations
    Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Besthesda, Maryland, USA
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  • Griff T. Ross
    Affiliations
    Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Besthesda, Maryland, USA
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      Abstract

      With antiserum to the β-subunit of human chorionic gonadotropin (HCG), we have developed a radioimmunoassay which selectively measures HCG in samples containing both human pituitary luteinizing hormone (HLH) and HCG. High HLH levels observed in samples obtained at the midcycle peak or from castrate patients do not cause significant inhibition in the specific HCG radioimmunoassay. The sensitivity of the assay is sufficient for distinguishing HCG from follicular and luteal phase HLH levels. This specific HCG radioimmunoassay is ideal for following serum HCG levels in patients under going chemotherapy for HCG-secreting tumors as well as for follow-up of patients after termination of molar pregnancies. In addition, the sensitivity of the assay will permit earlier diagnosis of pregnancy which, in turn, would permit earlier therapeutic intervention if desired.
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