American Journal of Obstetrics & Gynecology
Volume 203, Issue 5 , Pages 494.e1-494.e6, November 2010

Two-component polyethylene glycol surgical sealant influence on intraperitoneal infection in a refined rodent model

Presented as a poster at the 36th Annual Meeting of the Society of Gynecologic Surgeon's Scientific Meeting, Tucson, AZ, April 12-14, 2010.

  • Kathleen E. Rodgers, PhD

      Affiliations

    • University of Southern California, Los Angeles, CA
    • ,
  • Florence G. Burleson, PhD

      Affiliations

    • BRT—Burleson Research Technologies, Inc, Morrisville, NC
    • ,
  • Gary R. Burleson, PhD

      Affiliations

    • BRT—Burleson Research Technologies, Inc, Morrisville, NC
    • ,
  • Martin J. Wolfsegger, PhD

      Affiliations

    • Baxter Healthcare Corporation, Deerfield, IL
    • ,
  • Kevin M. Lewis, DVM

      Affiliations

    • Baxter Healthcare Corporation, Deerfield, IL
    • Corresponding Author InformationReprints: Kevin M. Lewis, DVM, Baxter Healthcare Corporation, One Baxter Parkway, Deerfield, IL 60015
    • ,
  • Heinz Redl, PhD

      Affiliations

    • Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Cluster for Tissue Regeneration, Vienna, Austria

Received 5 June 2010; accepted 21 June 2010. published online 02 September 2010.

Objective

This study determined the influence of a 2-component polyethylene glycol surgical sealant (Coseal) as an adhesion prevention device on sepsis-related mortality and/or systemic bacterial translocation to the spleen.

Study Design

A bacterial inoculum and telemetry probe were implanted in 50 treated and 49 untreated rats. Telemetry probes monitored core-body temperature to determine time of death. Spleens were collected on day 3 for quantitative bacteriology of Escherichia coli and Bacteroides fragilis.

Results

Median survival time and mortality of treated rats (37.0 hours, 54.0%) were noninferior to untreated rats (47.5 hours, 55.1%). Median E coli titers in treated rats (2.24 log colony forming units/spleen) were significantly less than untreated rats (4.32 log colony forming units/spleen). B fragilis titers were not different.

Conclusion

This study demonstrates intraperitoneal administration of a 2-component polyethylene glycol surgical sealant as an adhesion prevention device does not alter time to death or sepsis-related mortality and/or systemic bacterial translocation to the spleen.

Key words: adhesion barrier, animal model, Coseal, intraperitoneal infection, rodent model

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 Drs Kevin Lewis and Martin Wolfsegger are employees of and receive an annual salary from Baxter Healthcare Corporation, Deerfield, IL. Drs Kathy Rodgers and Heinz Redl are consultants to, and each received a consultant fee from, Baxter Healthcare Corporation. Drs Florence Burleson and Gary Burleson of BRT-Burleson Research Technologies, Inc. were contracted by Baxter Healthcare Corporation to perform the study. Baxter Healthcare Corporation funded the study.

 Cite this article as: Rodgers KE, Burleson FG, Burleson GR, et al. Two-component polyethylene glycol surgical sealant influence on intraperitoneal infection in a refined rodent model. Am J Obstet Gynecol 2010;203:494.e1-6.

PII: S0002-9378(10)00836-7

doi:10.1016/j.ajog.2010.06.056

American Journal of Obstetrics & Gynecology
Volume 203, Issue 5 , Pages 494.e1-494.e6, November 2010

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