American Journal of Obstetrics & Gynecology
Volume 203, Issue 4 , Pages 365.e1-365.e6, October 2010

Maternal blood mitochondrial DNA content during normal and intrauterine growth restricted (IUGR) pregnancy

Presented at the 56th Annual Scientific Meeting of the Society for Gynecologic Investigations, Glasgow, United Kingdom, March 19-21, 2009.

  • Francesca Colleoni, PhD

      Affiliations

    • Unit of Obstetrics and Gynecology, Department of Clinical Sciences “L.Sacco” and Center for Fetal Research Giorgio Pardi, University of Milan, Milan, Italy
    • ,
  • Debora Lattuada, PhD

      Affiliations

    • Department of Mother and Infant Sciences, University of Milan, Foundation Istituto di Ricovero e Cura a Carattere Scientifico Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy
    • ,
  • Ambra Garretto, MD

      Affiliations

    • Unit of Obstetrics and Gynecology, Department of Clinical Sciences “L.Sacco” and Center for Fetal Research Giorgio Pardi, University of Milan, Milan, Italy
    • Division of Obstetrics and Gynecology, San Paolo Hospital, Milan, Italy
    • ,
  • Maddalena Massari, MD

      Affiliations

    • Unit of Obstetrics and Gynecology, Department of Clinical Sciences “L.Sacco” and Center for Fetal Research Giorgio Pardi, University of Milan, Milan, Italy
    • ,
  • Chiara Mandò, PhD

      Affiliations

    • Unit of Obstetrics and Gynecology, Department of Clinical Sciences “L.Sacco” and Center for Fetal Research Giorgio Pardi, University of Milan, Milan, Italy
    • ,
  • Edgardo Somigliana, MD

      Affiliations

    • Department of Mother and Infant Sciences, University of Milan, Foundation Istituto di Ricovero e Cura a Carattere Scientifico Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy
    • ,
  • Irene Cetin, MD

      Affiliations

    • Unit of Obstetrics and Gynecology, Department of Clinical Sciences “L.Sacco” and Center for Fetal Research Giorgio Pardi, University of Milan, Milan, Italy
    • Corresponding Author InformationReprints: Irene Cetin, MD, Unit of Obstetrics and Gynecology, Department of Clinical Sciences “L.Sacco” and Center for Fetal Research Giorgio Pardi, University of Milan, Italy; via G.B. Grassi, 74 20157 Milan, Italy

Received 11 March 2010; received in revised form 4 May 2010; accepted 18 May 2010. published online 09 July 2010.

Objective

We investigated mitochondrial DNA (mtDNA) content in the maternal circulation of normal pregnancies of different gestational ages and in pregnancies complicated by intrauterine growth restriction (IUGR).

Study Design

We examined 70 maternal blood samples: 13 nonpregnant women; 45 normal pregnancies, divided into the 3 trimesters; and 12 pregnancies complicated by IUGR. MtDNA content was determined by real-time quantitative polymerase chain reaction, using a genomic control and a target gene.

Results

A highly significant progressive reduction in circulating mtDNA was observed in pregnant women of first, second, and third trimesters and compared to nonpregnant women (mean value: 237, 188, 144, and 283, respectively; P < .001). Moreover, mtDNA was significantly increased in women carrying IUGR fetuses compared to women with normal pregnancies (430 vs 144; P < .001).

Conclusion

MtDNA could provide new insight into the mechanisms that occur during physiological gestation. Furthermore, mtDNA content may help recognize the IUGR disease in pregnancy.

Key words: intrauterine growth restriction, maternal blood, mitochondrial DNA, pregnancy

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 Supported by the Sixth Framework Programme of the European Union (Pregenesys, Contract No. LSHB CT 2006 037244), Ministero della Salute (finalized project “Role of new inflammatory molecules in pregnancy pathologies and in maternal-neonatal health”) and by Associazione Italiana per lo Studio delle Malformazioni.

 Cite this article as: Colleoni F, Lattuada D, Garretto A, et al. Maternal blood mitochondrial DNA content during normal and intrauterine growth restricted (IUGR) pregnancy. Am J Obstet Gynecol 2010;203:365.e1-6.

PII: S0002-9378(10)00670-8

doi:10.1016/j.ajog.2010.05.027

American Journal of Obstetrics & Gynecology
Volume 203, Issue 4 , Pages 365.e1-365.e6, October 2010

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