American Journal of Obstetrics & Gynecology
Volume 203, Issue 1 , Pages 32.e1-32.e8 , July 2010

Noninvasive diagnosis of intraamniotic infection: proteomic biomarkers in vaginal fluid

Presented in part at the 25th Annual Meeting of the Society for Maternal-Fetal Medicine, San Francisco, CA, Feb. 5-10, 2007.

  • Jane Hitti, MD

      Affiliations

    • Department of Obstetrics/Gynecology, University of Washington, Seattle, WA
    • ,
  • Jodi A. Lapidus, PhD

      Affiliations

    • Department of Public Health and Preventive Medicine, Oregon Health & Science University, Portland, OR
    • ProteoGenix Inc., Beaverton, OR
    • ,
  • Xinfang Lu, MS

      Affiliations

    • ProteoGenix Inc., Beaverton, OR
    • ,
  • Ashok P. Reddy, PhD

      Affiliations

    • ProteoGenix Inc., Beaverton, OR
    • ,
  • Thomas Jacob, PhD

      Affiliations

    • ProteoGenix Inc., Beaverton, OR
    • ,
  • Surendra Dasari, PhD

      Affiliations

    • ProteoGenix Inc., Beaverton, OR
    • ,
  • David A. Eschenbach, MD

      Affiliations

    • Department of Obstetrics/Gynecology, University of Washington, Seattle, WA
    • ,
  • Michael G. Gravett, MD

      Affiliations

    • Department of Obstetrics/Gynecology, University of Washington, Seattle, WA
    • ProteoGenix Inc., Beaverton, OR
    • ,
  • Srinivasa R. Nagalla, MD

      Affiliations

    • Department of Pediatrics, Oregon Health & Science University, Portland, OR
    • ProteoGenix Inc., Beaverton, OR

Received 17 September 2009 ,Revised 15 January 2010 ,Accepted 18 March 2010.

References 

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  5. Hillier SL, Witkin SS, Krohn MA, Watts DH, Kiviat NB, Eschenbach DA. The relationship of amniotic fluid cytokines and preterm delivery, amniotic fluid infection, histologic chorioamnionitis, and chorioamnion infection. Obstet Gynecol. 1993;81:941–948
  6. Romero R, Yoon BH, Mazor M, et al. The diagnostic and prognostic value of amniotic fluid white blood cell count, glucose, interleukin-6, and gram stain in patients with preterm labor and intact membranes. Am J Obstet Gynecol. 1993;169:805–816
  7. Yoon BH, Jun JK, Romero R, et al. Amniotic fluid inflammatory cytokines (interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha), neonatal brain white matter lesions, and cerebral palsy. Am J Obstet Gynecol. 1997;177:19–26
  8. Dasari S, Pereira L, Reddy AP, et al. Comprehensive proteomic analysis of human cervical-vaginal fluid. J Proteome Res. 2007;6:1258–1268
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  10. Pereira L, Reddy AP, Jacob T, et al. Identification of novel protein biomarkers of preterm birth in human cervical-vaginal fluid. J Proteome Res. 2007;6:1269–1276
  11. Hitti J, Hillier SL, Agnew KJ, Krohn MA, Reisner DP, Eschenbach DA. Vaginal indicators of amniotic fluid infection in preterm labor. Obstet Gynecol. 2001;97:211–219
  12. Hitti J, Riley DE, Krohn MA, et al. Broad-spectrum bacterial rDNA polymerase chain reaction assay for detecting amniotic fluid infection among women in premature labor. Clin Infect Dis. 1997;24:1228–1232
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  18. Lockwood CJ, Senyei AE, Dische MR, et al. Fetal fibronectin in cervical and vaginal secretions as a predictor of preterm delivery. N Engl J Med. 1991;325:669–674
  19. Goldenberg RL, Mercer BM, Meis PJ, Copper RL, Das A, McNellis D. The preterm prediction study: fetal fibronectin testing and spontaneous preterm birth: NICHD Maternal Fetal Medicine Units Network. Obstet Gynecol. 1996;87:643–648
  20. Athayde N, Romero R, Gomez R, et al. Matrix metalloproteinases-9 in preterm and term human parturition. J Matern Fetal Med. 1999;8:213–219
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 Supported by National Institutes of Health Grant no. AI31871. These analyses were supported by ProteoGenix, Inc.

 Oregon Health & Science University and Drs Gravett, Nagalla, and Lapidus have a significant financial interest in ProteoGenix, Inc, which is a company that may have a commercial interest in the results of this research and technology. This potential conflict of interest has been reviewed, and a management plan has been approved by the Oregon Health & Science University Integrity Program Oversight Council and by the University of Washington Office of Technology Transfer. ProteoGenix provided the technical assays and proteomic analysis. Article preparation and submission was independent of the sponsor.

 Reprints not available from the authors.

 Cite this article as: Hitti J, Lapidus JA, Lu X, et al. Noninvasive diagnosis of intraamniotic infection: proteomic biomarkers in vaginal fluid. Am J Obstet Gynecol 2010;203:32.e1-8.

PII: S0002-9378(10)00356-X

doi: 10.1016/j.ajog.2010.03.037

American Journal of Obstetrics & Gynecology
Volume 203, Issue 1 , Pages 32.e1-32.e8 , July 2010

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