American Journal of Obstetrics & Gynecology
Volume 202, Issue 6 , Pages 555.e1-555.e10, June 2010

Insights into the multifactorial nature of preterm birth: proteomic profiling of the maternal serum glycoproteome and maternal serum peptidome among women in preterm labor

Presented in abstract form at the 28th Annual Meeting of the Society for Maternal-Fetal Medicine, Dallas, TX, Jan. 28-Feb. 2, 2008.

  • Leonardo Pereira, MD, MCR

      Affiliations

    • Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR
    • Corresponding Author InformationReprints: Leonardo Pereira, MD, Division of Maternal Fetal Medicine, 3181 SW Sam Jackson Park Rd., Mailcode L458, Oregon Health & Science University, Portland, OR 97239
    • ,
  • Ashok P. Reddy, PhD

      Affiliations

    • Diabetomics, LLC, Portland, OR
    • ,
  • Amanda L. Alexander, BS

      Affiliations

    • Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR
    • ,
  • Xinfang Lu, MS

      Affiliations

    • Proteogenix, Inc, Beaverton, OR
    • ,
  • Jodi A. Lapidus, PhD

      Affiliations

    • Division of Biostatistics, Department of Public Health and Preventive Medicine, Oregon Health & Science University, Portland, OR
    • Proteogenix, Inc, Beaverton, OR
    • ,
  • Michael G. Gravett, MD

      Affiliations

    • Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Washington School of Medicine, Seattle, WA
    • ,
  • Srinivasa R. Nagalla, MD

      Affiliations

    • Diabetomics, LLC, Portland, OR

Received 2 July 2009; received in revised form 24 December 2009; accepted 17 February 2010. published online 22 April 2010.

Objective

The purpose of this study was to identify peptide classifiers that predict spontaneous preterm birth (SPTB) among women in preterm labor (PTL) and to demonstrate specific protein pathways that are activated in PTL.

Study Design

Serum from 110 women with PTL between 20 weeks and 33 weeks 6 days of gestation was subjected to glycoprotein purification, matrix-assisted laser desorption ionization time-of-flight mass spectrometry peptide profiling, 2-dimensional liquid chromatography tandem mass spectrometry, and pathway analysis. Women were divided into 2 groups: delivery at <34 weeks' gestation (SPTB group) and delivery at ≥34 weeks' gestation (PTL group).

Results

Twenty-three peptide masses were identified that discriminated PTL from SPTB in 97% of cases. Fifty-two proteins were present differentially between PTL and SPTB; 48 of 52 proteins were classified into 1 of 4 functional pathways that were involved with PTL: (1) complement/coagulation cascade, (2) inflammation/immune response, (3) fetal-placental development, and (4) extracellular matrix proteins.

Conclusion

Among women in PTL, proteomic analysis of serum peptides and glycoproteins classifies women who will deliver preterm and identifies specific protein pathways at work among individuals with “idiopathic” PTL.

Key words: preterm birth, preterm labor, proteomics, peptide, glycoprotein

 

 Supported by the Oregon Clinical and Translational Research Institute, Grant no. UL1 RR024140 from the National Center for Research Resources, a component of the National Institutes of Health, and National Institutes of Health Roadmap for Medical Research.

 ProteoGenix, Inc, provided the laboratory and equipment that made this research possible. Oregon Health & Science University and Drs Gravett, Nagalla, and Lapidus have a significant financial interest in ProteoGenix, Inc, a company that may have a commercial interest in the results of this research and technology. This potential conflict of interest has been reviewed and a management plan has been approved by the Oregon Health & Science University Integrity Program Oversight Council and by the University of Washington Office of Technology Transfer.

 Cite this article as: Pereira L, Reddy AP, Alexander AL, et al. Insights into the multifactorial nature of preterm birth: proteomic profiling of the maternal serum glycoproteome and maternal serum peptidome among women in preterm labor. Am J Obstet Gynecol 2010;202:555.e1-10.

PII: S0002-9378(10)00274-7

doi:10.1016/j.ajog.2010.02.048

American Journal of Obstetrics & Gynecology
Volume 202, Issue 6 , Pages 555.e1-555.e10, June 2010