Amplification of the chromosome 3q26 region shows high negative predictive value for nonmalignant transformation of LSIL cytologic finding
Received 7 July 2009; received in revised form 14 October 2009; accepted 15 December 2009. published online 22 February 2010.
Objective
The chromosome 3q26 region is a biomarker for cervical cancer. Women with low-grade squamous intraepithelial lesions (LSIL) currently are referred for immediate colposcopy. The objective of this study was to determine the negative predictive value of the 3q26 amplification test for the persistence or regression of LSIL.
Study Design
Archival thin layer cytologic slides of 47 women (14–67 years old) with LSIL were linked to histologic and cytologic end points. To determine 3q status, the slides were hybridized for the chromosome 3q26 region and for the centromere of chromosome 7, as a control, with the use of the standard fluorescent in situ hybridization methods.
Results
The negative predictive value of 3q26 gain for the development of cervical intraepithelial neoplasia grade 2/3 within 1 year was 93% (95% confidence interval, 68– 100); after 21 months, its negative predictive value was 100% (95% confidence interval, 29–100).
Conclusion
The 3q26 gain might help identify women with LSIL who do not need colposcopy.
bDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University, New York, NY
cNew England Pathology Associates, Mercy Medical Center, Springfield, MA
Reprints: Michael W. Kilpatrick, PhD, Ikonisys Inc., 5 Science Park, New Haven, CT 06511
Authorship and contribution to the article is limited to the 5 authors indicated. There was no outside funding or technical assistance with the production of this article.
Cite this article as: Jalali GR, Herzog TJ, Dziura B, et al. Amplification of the chromosome 3q26 region shows high negative predictive value for nonmalignant transformation of LSIL cytology. Am J Obstet Gynecol 2010;202:581.e1-5.
ABSTRACT