830: The effect of serum from women with antiphospholipid syndrome (APLS) on JAR (trophoblast-like) cell line invasion and matrix metalloproteinase (MMP) secretion

Article Outline

• Objective
• Study Design
• Results
• Conclusion
• Copyright

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Objective 

To assess the effect of serum obtained from women with APLS on invasion capacity and MMP2 and MMP9 secretion of JAR human choriocarcinoma cells.

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Study Design 

Blood was collected from 12 APLS and 10 healthy non-pregnant women. APLS was diagnosed and confirmed in women with a history of recurrent miscarriages, late fetal death or thrombosis, and with positive anticardiolipin antibodies and/or lupus anticoagulant at least twice 12 weeks apart. Controls were healthy women with a normal obstetric history and a negative thrombophilia workup. The JAR human choriocarcinoma cell line was cultured in the presence of 10% serum. Cell invasion and MMP (2 and 9) secretion were measured using the Matrigel invasion assay, and gel zymography, respectively.

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Results 

JAR invasion was reduced by 21% in the APLS group (68+5%) compared to controls (89+6%) (P<0.01). MMP-2 secretion was reduced in the APLS group by 69% (319+127 study vs 1029+149 controls optical density units, P<0.03). MMP-9 secretion was not significantly different between the groups (228+11 study vs 637+183 controls optical density units, P=0.07).

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Conclusion 

Serum obtained from women with APLS has an inhibitory effect on JAR trophoblast cell invasiveness and MMP-2 secretion. This effect may be involved in the early pathologic implantation associated with the pathogenesis of APLS.