A maternal high-fat diet is accompanied by alterations in the fetal primate metabolome
Presented at the 29th Annual Meeting of the Society for Maternal-Fetal Medicine, San Diego, CA, Jan. 26-31, 2009.
Received 21 February 2009; received in revised form 22 April 2009; accepted 16 June 2009.
Objective
To characterize the serum metabolome of a primate model of in utero high-fat exposure.
Study Design
Serum from maternal and fetal (e130) macaque monkeys exposed to either a high-fat or control diet were analyzed by gas chromatography-mass spectrometry. Multivariate data analysis was performed to reduce the generated data set. Candidate metabolites were further analyzed for significance by using the analysis of variance and comparative t tests.
Results
Approximately 1300 chromatographic features were detected. Through multivariate data analysis this number was reduced to 60 possible metabolites. With the use of comparative t tests, 22 metabolites had statistical significance (P < .05) over the entire study. By virtue of maternal high-fat diet alone, fetal phenotypic differences are accompanied by altered metabolite concentrations of 7 metabolites (P < .05).
Conclusion
In utero high-fat diet exposure is associated with an altered fetal epigenome and parlays a characteristic modification in the fetal metabolite profile.
aMetabolomics Core Research Facility, University of Utah Health Sciences, Salt Lake City, UT
bDivision of Neonatology, Department of Pediatrics, University of Utah Health Sciences, Salt Lake City, UT
cOregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR
dDivision of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, TX
Reprints not available from the authors.
Support for this work was provided by the National Institutes of Health (NIH) Director New Innovator Pioneer Award DP21DP2OD001500-01 (K.A.T.), and NIH Grants 1R01DK080558 (R.H.L. and K.A.T.), DK60685-0351 (K.L.G.), 1R01DK079194 (K.L.G.), and RR00163 (K.L.G.-ONPRC).
Cite this article as: Cox J, Williams S, Grove K, et al. A maternal high-fat diet is accompanied by alterations in the fetal primate metabolome. Am J Obstet Gynecol 2009;201:281.e1-9.
ABSTRACT
A maternal high-fat diet is accompanied by alterations in the fetal primate metabolome