American Journal of Obstetrics & Gynecology
Volume 195, Issue 3 , Pages 822-828, September 2006

Uterine transcriptomes of bacteria-induced and ovariectomy-induced preterm labor in mice are characterized by differential expression of arachidonate metabolism genes

  • Ramsi Haddad, PhD

      Affiliations

    • Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, and Detroit, MI
    • ,
  • Barbara R. Gould, PhD

      Affiliations

    • Department of Pharmacology and Therapeutics, McGill University, Montréal, Québec, Canada
    • ,
  • Roberto Romero, MD

      Affiliations

    • Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, and Detroit, MI
    • Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI
    • ,
  • Gerard Tromp, PhD

      Affiliations

    • Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, and Detroit, MI
    • Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI
    • ,
  • Riaz Farookhi, PhD

      Affiliations

    • Department of Physiology, McGill University, Montréal, Québec, Canada
    • ,
  • Sam S. Edwin, PhD

      Affiliations

    • Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, and Detroit, MI
    • ,
  • Mi Ran Kim, MS

      Affiliations

    • Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, and Detroit, MI
    • ,
  • Hans H. Zingg, MD, PhD

      Affiliations

    • Department of Pharmacology and Therapeutics, McGill University, Montréal, Québec, Canada

Received 10 March 2006; received in revised form 25 May 2006; accepted 15 June 2006.

Objective

The purpose of this study was to identify changes in gene expression that are associated with preterm labor induced by either bacteria or ovariectomy.

Study design

Pregnant mice (14.5 days of gestation) were allocated to: (1) intrauterine injection of heat-inactivated Escherichia coli; (2) media alone; (3) ovariectomy; or (4) sham operation. The uterine transcriptome was studied with photolithographic, very short oligonucleotide-based microarrays, and arachidonate metabolism genes were assayed with quantitative reverse transcriptase–polymerase chain reaction. Significance was determined by analysis of variance.

Results

Microarray-based gene expression changes in the arachidonate metabolism pathway are associated globally with bacteria-induced preterm labor (P ≤ .0031) and ovariectomy-induced preterm labor (P ≤ .00036). Quantitative real-time reverse transcriptase–polymerase chain reaction measurements demonstrated that bacteria-induced preterm labor substantially increased the expression of genes involved in prostaglandin synthesis. In contrast, ovariectomy-induced preterm labor increased the expression of genes involved in lipoxin, leukotriene, and hydroxyeicosatetraenoic acid synthesis.

Conclusion

Bacteria-induced and ovariectomy-induced preterm labor each express a different balance of genes that are required for the synthesis of prostaglandins, lipoxins, leukotrienes, and hydroxyeicosatetraenoic acids.

Key words: Mouse, Cyclooxygenase pathway, Lipoxygenase pathway, Parturition, Infection, Progesterone

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 Supported in part by the Intramural Research Program of the National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services.Presented at the 26th Annual Meeting of the Society for Maternal Fetal Medicine, January 30-February 4, 2006, Miami, FL.Reprints not available. Address correspondence to Roberto Romero, MD, Hutzel Women's Hospital, 3990 John R, 4th Floor, Detroit, MI 48201-2018 or Ramsi Haddad, PhD, Translational Oncogenomics Laboratory, Karmanos Cancer Institute, Detroit, MI 48201.

PII: S0002-9378(06)00808-8

doi:10.1016/j.ajog.2006.06.053

American Journal of Obstetrics & Gynecology
Volume 195, Issue 3 , Pages 822-828, September 2006

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