American Journal of Obstetrics & Gynecology
Volume 202, Issue 5 , Pages 485.e1-485.e9, May 2010

Effect of hormone replacement and selective estrogen receptor modulators (SERMs) on the biomechanics and biochemistry of pelvic support ligaments in the cynomolgus monkey (Macaca fascicularis)

Presented in part at the 34th Annual Scientific Meeting of the Society of Gynecologic Surgeons, Savannah, GA, April 12-17, 2008, and in full at the 30th Annual Scientific Meeting of the American Urogynecologic Society, Hollywood, FL, Sept. 24-26, 2009.

  • Azin Shahryarinejad, MD, MPH

      Affiliations

    • Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology, Keck School of Medicine at USC, Los Angeles, CA
    • ,
  • Thomas R. Gardner, MCE

      Affiliations

    • Center for Orthopaedic Research, Department of Orthopaedic Surgery, Columbia University College of Physicians and Surgeons, New York, NY
    • ,
  • J. Mark Cline, DVM, PhD

      Affiliations

    • Section on Comparative Medicine, Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC
    • ,
  • William N. Levine, MD

      Affiliations

    • Center for Orthopaedic Research, Department of Orthopaedic Surgery, Columbia University College of Physicians and Surgeons, New York, NY
    • ,
  • Haley A. Bunting, MS

      Affiliations

    • Center for Orthopaedic Research, Department of Orthopaedic Surgery, Columbia University College of Physicians and Surgeons, New York, NY
    • ,
  • Michael D. Brodman, MD

      Affiliations

    • Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology, Mount Sinai School of Medicine, New York, NY
    • ,
  • Charles J. Ascher-Walsh, MD

      Affiliations

    • Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology, Mount Sinai School of Medicine, New York, NY
    • ,
  • Richard J. Scotti, MD

      Affiliations

    • Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology, Keck School of Medicine at USC, Los Angeles, CA
    • ,
  • Michael D. Vardy, MD

      Affiliations

    • Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology, Mount Sinai School of Medicine, New York, NY

Received 4 August 2009; received in revised form 10 November 2009; accepted 24 January 2010.

Objective

To evaluate the effect of selective estrogen receptor modulators and ethinyl estradiol on the biomechanical and biochemical properties of the uterosacral and round ligaments in the monkey model of menopause.

Study Design

A randomized, double-blind, placebo-controlled study on 11 female macaque monkeys. Ovariectomized monkeys received 12 weeks of placebo, raloxifene, tamoxifen, or ethinyl estradiol. Biomechanical step-strain testing and real-time polymerase chain reaction was performed on the uterosacral and round ligaments.

Results

Tamoxifen and raloxifene uterosacrals expressed differing collagen I /III receptor density ratios, but both selective estrogen receptor modulators showed decreased tensile stiffness compared to ethinyl estradiol and controls.

Conclusion

These findings support a possible effect of selective estrogen receptor modulators on biomechanical and biochemical properties of uterosacrals. This may play a role in pelvic organ prolapse.

Key words: macaca fascicularis, pelvic organ prolapse, round ligament, SERMs, uterosacral ligament

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 Cite this article as: Shahryarinejad A, Gardner TR, Cline JM, et al. Effect of hormone replacement and selective estrogen receptor modulators (SERMs) on the biomechanics and biochemistry of pelvic support ligaments in the cynomolgus monkey (Macaca fascicularis). Am J Obstet Gynecol 2010;202:485.e1-9.

 Reprints not available from the authors.

 This study was funded by the American College of Obstetricians and Gynecologists ACOG/Solvay Research Award in Menopause. The parent study was supported in part by Johnson and Johnson Pharmaceutical Research and Development, LLC.

PII: S0002-9378(10)00104-3

doi:10.1016/j.ajog.2010.01.074

American Journal of Obstetrics & Gynecology
Volume 202, Issue 5 , Pages 485.e1-485.e9, May 2010

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