Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice
Received 31 March 2009; received in revised form 31 August 2009; accepted 28 October 2009. published online 14 January 2010.
Objective
The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model.
Study Design
ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment.
Results
Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05).
Conclusion
EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.
Center for Women's Health Research, Department of Obstetrics and Gynecology, Meharry Medical College, Nashville, TN
Reprints: Ayman Al-Hendy, MD, PhD, Professor and Vice Chair, Center for Women's Health Research, Department of Obstetrics and Gynecology, Meharry Medical College, 1005 Dr D. B. Todd Jr Blvd., Nashville, TN 37208
Cite this article as: Zhang D, Al-Hendy M, Richard-Davis G, et al. Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. Am J Obstet Gynecol 2010;202:289.e1-9.
ABSTRACT
Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice