American Journal of Obstetrics & Gynecology
Volume 202, Issue 3 , Pages 289.e1-289.e9, March 2010

Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice

Center for Women's Health Research, Department of Obstetrics and Gynecology, Meharry Medical College, Nashville, TN

Received 31 March 2009; received in revised form 31 August 2009; accepted 28 October 2009. published online 14 January 2010.

Objective

The purpose of this study was to investigate the effect of epigallocatechin gallate (EGCG) on rat leiomyoma (ELT3) cells in vitro and in a nude mice model.

Study Design

ELT3 cells were treated with various concentrations of EGCG. Cell proliferation, proliferation cell nuclear antigen (PCNA), and cyclin-dependent kinase 4 (Cdk4) protein levels were evaluated. ELT3 cells were inoculated subcutaneously in female athymic nude mice. Animals were fed 1.25 mg EGCG (in drinking water)/mouse/day. Tumors were collected and evaluated at 4 and 8 weeks after the treatment.

Results

Inhibitory effect of EGCG (200 μmol/L) on ELT3 cells was observed after 24 hours of treatment (P < .05). At ≥50 μmol/L, EGCG significantly decreased PCNA and Cdk4 protein levels (P < .05). In vivo, EGCG treatment dramatically reduced the volume and weight of tumors at 4 and 8 weeks after the treatment (P < .05). The PCNA and Cdk4 protein levels were significantly reduced in the EGCG-treated group (P < .05).

Conclusion

EGCG effectively inhibits proliferation and induces apoptosis in rat ELT3 uterine leiomyoma cells in vitro and in vivo.

Key words: apoptosis, epigallocatechin gallate (EGCG), green tea extract, inhibitory effect, uterine leiomyoma

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 This study was supported by Research Center for Minority Institutes (RCMI) Grant G12 RR03032 and National Institutes of Health/National Institute of Child Health and Human Development 1 R01 HD046228 (A.A.).

 Cite this article as: Zhang D, Al-Hendy M, Richard-Davis G, et al. Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. Am J Obstet Gynecol 2010;202:289.e1-9.

PII: S0002-9378(09)02102-4

doi:10.1016/j.ajog.2009.10.885

American Journal of Obstetrics & Gynecology
Volume 202, Issue 3 , Pages 289.e1-289.e9, March 2010

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